Claim · model-written summary
B5 medium raised C2 RA content to 40.8 mg/g DW, fourfold above MS medium.
- Taxon
- Perovskia atriplicifolia
Salvia yangiie0 - Material
- C2e1
- Treatment
- Gamborg (B5) mediume1
- Outcome
- RA contente1
- Direction
- enhancede1
- Value
- 40.8 mg/g dry weight (DW)e1
- Qualifier
- fourfold increasee1
- Comparator
- Murashige and Skoog (MS) mediume1
Not reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSetting
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Perovskia atriplicifolia hairy root cultures”
e1“Gamborg (B5) medium enhanced RA content, achieving 38.3 and 40.8 mg/g dry weight (DW) for clones C1 and C2, respectively, representing a fourfold increase compared to the least favorable Murashige and Skoog (MS) medium”
Title
Enhancement of Rosmarinic Acid Production in Hairy Root Cultures of Perovskia atriplicifolia Benth
Renata Grąbkowska; Marta Krzemińska; Katarzyna Gaweda-Walerych; et al. International Journal of Molecular Sciences 2025. doi:10.3390/ijms26073187
Other claims from this paper (11)
- Method / resource Perovskia hairy root cultures were successfully established using Rhizobium rhizogenes.
- Observation PCR targeting rol and aux genes confirmed transformation.
- Observation R. rhizogenes A4 transformed roots at 41.3%, higher than ATCC 15834 at 30.2%.
- Method / resource Eight transformed root clones C1–C8 were established.
- Observation The clones varied significantly in biomass accumulation and phenolic acid production.
- Association RA production was most strongly correlated with PAL, RAS, and CYP98A14 expression.
- Observation Clones formed three clusters by growth, metabolite content, and gene expression.
- Method / resource C1 and C2 were selected for further experiments for high RA, polyphenol content, and productivity.
- Intervention effect WP medium gave greatest biomass accumulation with growth index over 13.
- Intervention effect SH medium gave greatest biomass accumulation with growth index over 13.
- Intervention effect B5 medium raised C1 RA content to 38.3 mg/g DW, fourfold above MS medium.