Claim · model-written summary
NH(4)Cl (50 mm) lowered urease induction by 50%
- Taxon
- soybean
Glycine maxe2 - Treatment
- NH(4)Cl (50 mm)e1
- Outcome
- urease inductione1
- Direction
- lowerse1
- Value
- 50%e1
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“NH(4)Cl (50 mm) lowers urease induction by 50% whereas 50 mm methylammonium chloride results in more drastic reductions in urea-stimulated urease levels”
e2“Cultured soybean (Glycine max, Kanrich variety) cells grow with 25 mm urea as the sole nitrogen source but at a slower rate than with the Murashige and Skoog (MS) (Physiol. Plant. 15: 473-497, 1962) nitrogen source of 18.8 mm KNO(3) and 20.6 mm NH(4)NO(3)”
Title
Nitrogen Metabolism in Soybean Tissue Culture
Joseph Carmine Polacco PLANT PHYSIOLOGY 1976. doi:10.1104/pp.58.3.350
Other claims from this paper (15)
- Intervention effect Soybean cells grew more slowly with 25 mm urea as sole nitrogen source than with the MS nitrogen source
- Intervention effect Growth with urea was restricted by 18.8 mm nitrate, 50 mm methylammonia, 10 mm citrate or 100 µM hydroxyurea
- Intervention effect Callus did not grow with 1 mm urea plus 18.8 mm KNO3
- Intervention effect Urease levels decreased 80% within two divisions after transfer from MS to 1 mm urea plus 18.8 mm KNO3
- Mechanism Hydroxyurea is a potent inhibitor of soybean urease, which appears to be the basis for its inhibition of urea utilization by callus cells
- Intervention effect Urease levels increased in the presence of urea and MS nitrogen source, by 10 to 20 times more with urea
- Intervention effect 50 mm methylammonium chloride produced more drastic reductions in urea-stimulated urease levels than NH4Cl
- Intervention effect Citrate (10 mm) completely blocked urease synthesis in the presence of urea
- Intervention effect Ammonia and methylammonia did not inhibit soybean urease
- Intervention effect Ammonia and methylammonia did not appreciably inhibit urea uptake by suspension cultures
- Mechanism Methylammonia inhibits urea utilization in cultured soybean cells, likely through its repressive effect on urease synthesis
- Intervention effect Citrate did not inhibit urease activity in vitro
- Intervention effect Citrate exhibited only a partial inhibition of urea uptake, 0-50% in several experiments
- Mechanism Citrate eliminated urease production in cultured soybean cells, likely through chelation of trace Ni(2+) in the growth medium
- Recommendation Urea-based selection schemes may be a model for improving seed protein quality by plant cell culture techniques