Claim · model-written summary
Cycloheximide treatment prolonged cell viability of light-grown Arabidopsis cultures.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- light-grown culturese5
- Treatment
- Cycloheximide treatmente5
- Outcome
- cell viabilitye5
- Direction
- prolongede5
Not reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana cell suspension cultures are model systems”
e5“Cycloheximide treatment of light-grown cultures prolonged cell viability and attenuated AL-PCD induction”
Title
S. M. Doyle; M. Diamond; P. F. McCabe Journal of Experimental Botany 2009. doi:10.1093/jxb/erp320
Other claims from this paper (12)
- Observation Light-grown Arabidopsis cell cultures contained well-developed, functional chloroplasts, while dark-grown cultures did not.
- Intervention effect Heat at 55 C induced significantly less AL-PCD in light-grown than in dark-grown Arabidopsis cultures.
- Intervention effect Heat at 55 C induced apoptotic-like programmed cell death in Arabidopsis cultures.
- Intervention effect Chloroplast-free light-grown cultures showed increased AL-PCD after heat treatment.
- Mechanism Chloroplasts affect AL-PCD induction in light-grown Arabidopsis cultures.
- Intervention effect Antioxidant treatment increased AL-PCD induction in light-grown Arabidopsis cultures.
- Mechanism Chloroplast-produced ROS may be involved in regulating AL-PCD.
- Intervention effect Cycloheximide treatment attenuated AL-PCD induction in light-grown Arabidopsis cultures.
- Intervention effect The cycloheximide effect was less pronounced in dark-grown cultures.
- Intervention effect The cycloheximide effect did not occur in antioxidant-treated light-grown cultures.
- Mechanism Light, chloroplasts, ROS and nuclear protein synthesis may interact during Arabidopsis AL-PCD.
- Recommendation Studies of AL-PCD should account for the time-point of observation and whether cells are light-grown and chloroplast-containing.