Claim · model-written summary
Heat at 55 C induced significantly less AL-PCD in light-grown than in dark-grown Arabidopsis cultures.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- light-grown culturese2
- Treatment
- Heat treatment at 55 degrees Ce2
- Outcome
- AL-PCDe2
- Direction
- lesse2
- Comparator
- dark-grown culturese2
Not reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana cell suspension cultures are model systems”
e2“Heat treatment at 55 degrees C induced apoptotic-like (AL)-PCD in the cultures, but light-grown cultures responded with significantly less AL-PCD than dark-grown cultures”
Title
S. M. Doyle; M. Diamond; P. F. McCabe Journal of Experimental Botany 2009. doi:10.1093/jxb/erp320
Other claims from this paper (12)
- Observation Light-grown Arabidopsis cell cultures contained well-developed, functional chloroplasts, while dark-grown cultures did not.
- Intervention effect Heat at 55 C induced apoptotic-like programmed cell death in Arabidopsis cultures.
- Intervention effect Chloroplast-free light-grown cultures showed increased AL-PCD after heat treatment.
- Mechanism Chloroplasts affect AL-PCD induction in light-grown Arabidopsis cultures.
- Intervention effect Antioxidant treatment increased AL-PCD induction in light-grown Arabidopsis cultures.
- Mechanism Chloroplast-produced ROS may be involved in regulating AL-PCD.
- Intervention effect Cycloheximide treatment prolonged cell viability of light-grown Arabidopsis cultures.
- Intervention effect Cycloheximide treatment attenuated AL-PCD induction in light-grown Arabidopsis cultures.
- Intervention effect The cycloheximide effect was less pronounced in dark-grown cultures.
- Intervention effect The cycloheximide effect did not occur in antioxidant-treated light-grown cultures.
- Mechanism Light, chloroplasts, ROS and nuclear protein synthesis may interact during Arabidopsis AL-PCD.
- Recommendation Studies of AL-PCD should account for the time-point of observation and whether cells are light-grown and chloroplast-containing.