Claim · model-written summary
No co-integration events were observed among 14 transgenic lines.
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Verbatim
t0“Sugarcane Biofactory”
e1“no events of co-integration were observed in a total of 14 transgenic lines recovered after bombardment with both constructs”
Title
Carbon Mobilisation and Utilisation in the Sugarcane Biofactory
Brian Patrick O’Neill Queensland's institutional digital repository (The University of Queensland) 2011
Other claims from this paper (18)
- Observation Sett internode pith sucrose was 99.0% depleted after 42 days of bud outgrowth.
- Observation Axillary bud outgrowth accumulated 2.66 g of new tissue after 42 days.
- Mechanism Carbon mobilisation was driven by the accumulation of new biomass, as shown by comparison with excised buds.
- Observation Stored sucrose was the only available carbon source of sufficient size at day zero for the biomass accumulation.
- Observation Isoleucine and leucine were depleted in shoot treatment setts at day 42.
- Mechanism Sucrose mobilisation is proposed to require a net sink-to-source transition in the storage parenchyma.
- Mechanism Sucrose mobilisation from culm storage parenchyma is proposed to require minimal metabolic investment.
- Mechanism The mechanism of sucrose mobilisation is proposed to be metabolically neutral.
- Observation Sucrose is mobilised from sett storage parenchyma via phloem to the growing shoot tissue.
- Association Sucrose synthase activity is down-regulated by sucrose mobilisation.
- Observation Storage parenchyma metabolism shifts from futile cycling to a more quiescent state during sucrose mobilisation.
- Observation Transgenic plants carrying each transgene singly were phenotypically indistinguishable from wild type.
- Intervention effect The RNAi transgene abrogated trehalase expression in many lines.
- Observation No decrease in trehalase activity was observed in the RNAi lines.
- Intervention effect A trehalase inhibitor decreased trehalase activity in sugarcane pith extracts by 80%.
- Intervention effect Trehalose biosynthesis activity was elevated in TPSP lines compared with RNAi lines, borderline significant.
- Association Trehalase activity was positively correlated with TPSP in RNAi transformants that expressed trehalase.
- Observation Soluble carbohydrate content was unaffected by the transgenes.