Claim · model-written summary
Trehalose biosynthesis activity was elevated in TPSP lines compared with RNAi lines, borderline significant.
- Taxon
- Sugarcanet0
- Treatment
- TPSPe1
- Outcome
- Trehalose biosynthesis activitye1
- Direction
- elevatede1
- Comparator
- RNAi linese1
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValueQualifier
Verbatim
t0“Sugarcane Biofactory”
e1“Trehalose biosynthesis activity in TPSP lines was elevated compared to RNAi lines and the difference bordered on significance (P = 0.080)”
Title
Carbon Mobilisation and Utilisation in the Sugarcane Biofactory
Brian Patrick O’Neill Queensland's institutional digital repository (The University of Queensland) 2011
Other claims from this paper (18)
- Observation Sett internode pith sucrose was 99.0% depleted after 42 days of bud outgrowth.
- Observation Axillary bud outgrowth accumulated 2.66 g of new tissue after 42 days.
- Mechanism Carbon mobilisation was driven by the accumulation of new biomass, as shown by comparison with excised buds.
- Observation Stored sucrose was the only available carbon source of sufficient size at day zero for the biomass accumulation.
- Observation Isoleucine and leucine were depleted in shoot treatment setts at day 42.
- Mechanism Sucrose mobilisation is proposed to require a net sink-to-source transition in the storage parenchyma.
- Mechanism Sucrose mobilisation from culm storage parenchyma is proposed to require minimal metabolic investment.
- Mechanism The mechanism of sucrose mobilisation is proposed to be metabolically neutral.
- Observation Sucrose is mobilised from sett storage parenchyma via phloem to the growing shoot tissue.
- Association Sucrose synthase activity is down-regulated by sucrose mobilisation.
- Observation Storage parenchyma metabolism shifts from futile cycling to a more quiescent state during sucrose mobilisation.
- Observation Transgenic plants carrying each transgene singly were phenotypically indistinguishable from wild type.
- Observation No co-integration events were observed among 14 transgenic lines.
- Intervention effect The RNAi transgene abrogated trehalase expression in many lines.
- Observation No decrease in trehalase activity was observed in the RNAi lines.
- Intervention effect A trehalase inhibitor decreased trehalase activity in sugarcane pith extracts by 80%.
- Association Trehalase activity was positively correlated with TPSP in RNAi transformants that expressed trehalase.
- Observation Soluble carbohydrate content was unaffected by the transgenes.