Claim · model-written summary
MBD9 is proposed to recognize nucleosome modifications and guide SWR1 to H2A.Z deposition sites.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- MBD9e0
- Outcome
- guides SWR1 to these sites for H2A.Z depositione0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we propose that MBD9 recognizes particular nucleosome modifications via its PHD- and Bromo-domains and thereby guides SWR1 to these sites for H2A.Z deposition”
t0“Arabidopsis genome”
Title
Paja Sijacic; Dylan H. Holder; Marko Bajic; et al. PLoS Genetics 2019. doi:10.1371/journal.pgen.1008326
Other claims from this paper (9)
- Observation The purified Arabidopsis complex contained all 11 subunits known from yeast SWR1 and mammalian SRCAP.
- Observation The complex also contained MBD9 and three Alfin1-like family proteins.
- Observation mbd9 mutant plants resembled arp6 mutants phenotypically.
- Mechanism MBD9 was needed for proper H2A.Z incorporation at thousands of discrete sites.
- Observation MBD9 preferentially interacted with acetylated H4 peptides and H3 peptides with specified methylation.
- Observation MBD9-dependent H2A.Z sites had a distinct histone modification profile.
- Observation MBD9 did not appear to be a core Arabidopsis SWR1 subunit.
- Observation The arp6;mbd9 double mutant displayed a synergistic phenotype.
- Mechanism The authors infer that MBD9 also has roles beyond H2A.Z deposition.