Claim · model-written summary
The highest sucrose concentration gave Fiona the highest microspore survival after 16 days.
- Taxon
- Brassica napus ssp. oleifera
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Brassica napus var. napuse0 - Material
- Fionae2
- Treatment
- 20% sucrosee1
- Outcome
- survivale1
- Direction
- higheste1
- Qualifier
- after 16 de1
- Comparator
- 8 %, 12%, 16%e1
Not reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“winter oil seed rape (Brassica napus ssp. oleifera)”
e1“A study of microspore survival and embryo induction in this cultivar on media containing either 8 %, 12%, 16% or 20% sucrose revealed the highest survival (after 16 d)”
e2“the winter cultivar (Fiona) revealed that the osmotic pressure of the supernatant of anther homogenates was equivalent to a solution of 17% sucrose.”
Title
Role of Sucrose in Microspore Embryo Production inBrassica napusssp.oleifera
J. M. DUNWELL; N. THURLING Journal of Experimental Botany 1985. doi:10.1093/jxb/36.9.1478
Other claims from this paper (8)
- Observation Winter cultivar Fiona anther homogenate supernatant had osmotic pressure equivalent to 17% sucrose.
- Intervention effect The highest sucrose concentration gave Fiona the greatest number of anthers with induced embryos after 42 days.
- Intervention effect Spring cultivars had much higher microspore survival at 16% than 8% sucrose.
- Intervention effect Spring cultivars had much higher embryo induction at 16% than 8% sucrose.
- Intervention effect Higher sucrose reduced variation in response among spring cultivars.
- Observation The benefit of 16% sucrose persisted across donor plant growth environments and pollen stages.
- Observation Macroscopic embryos emerged only from anthers grown at 8% sucrose.
- Recommendation The authors suggest transferring anthers from high to normal sucrose concentration during culture.