Claim · model-written summary
EDTA, sodium dihydrogen phosphate and ammonium metavanadate inhibited the wheat leaf phosphatase activity.
- Taxon
- wheat
Triticum aestivumt0 - Organ / stage
- leavest0
- Treatment
- EDTA,sodium dihydrogen phosphate and ammonium metavanadatee7
- Gene
- the enzymee7
- Outcome
- activity of the enzymee7
- Direction
- inhibitory effecte7
Not reportedMaterialCompoundInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the leaves of wheat”
e7“EDTA,sodium dihydrogen phosphate and ammonium metavanadate also presented an inhibitory effect on the activity of the enzyme”
Title
Biochemical properties of phosphatase in the leaves of wheat
Fei Meijuan; Chen Jian-sheng; Xiaoyun Wang Xibei zhiwu xuebao 2006
Other claims from this paper (12)
- Observation The wheat leaf phosphatase had a Km of 6.554 mmol/L for pNPP hydrolysis.
- Observation Vmax of the wheat leaf phosphatase in the pNPP hydrolysis was 0.774×10~3 U/(mg protein).
- Observation The optimal pH of the wheat leaf phosphatase was 6.8.
- Observation The optimal temperature of the wheat leaf phosphatase was 45℃ in a ten-minute thermal preservation.
- Observation The wheat leaf phosphatase lost 50% of its activity in 12 minutes of thermal preservation at 50 ℃.
- Observation The wheat leaf phosphatase lost 50% of its activity in 3.75 minutes of thermal preservation at 60℃.
- Observation The wheat leaf phosphatase lost 50% of its activity in 1.8 minutes of thermal preservation at 70℃.
- Intervention effect Mg2+ and Ca2+ were capable of activating the wheat leaf phosphatase.
- Intervention effect Zn2+, Cu2+ and Mn2+ inhibited the wheat leaf phosphatase activity.
- Observation The non-competitive inhibition constant of the wheat leaf phosphatase was 5.87 mmol/L.
- Observation The competitive inhibition constant of the wheat leaf phosphatase was 2.47 mmol/L.
- Observation The inhibition constant of the wheat leaf phosphatase for other inhibition types was 8.2 mmol/L.