Claim · model-written summary
The authors propose an electrostatic gate model to explain pH and substituent effects.
- Taxon
- horseradish
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Armoracia rusticanat0 - Gene
- horseradish peroxidaset0
- Outcome
- controls access of substrate to the active sitee0
- Qualifier
- negatively charged 'electrostatic gate'e0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“A model in which it is postulated that a negatively charged 'electrostatic gate' controls access of substrate to the active site and may also activate substrate within the active site, provides the most satisfactory explanation for both the present results and data from the literature.”
t0“horseradish peroxidase”
Title
D M Davies; P Jones; D Mantle Biochemical Journal 1976. doi:10.1042/bj1570247
Other claims from this paper (7)
- Association Compound I formation rate constants with peroxobenzoic acids declined as pH rose.
- Association Compound I formation with H2O2 was independent of pH.
- Observation Maximal Compound I formation rates with unhindered peroxobenzoic acids exceeded the H2O2 rate.
- Observation Bulky ortho substituents of peroxobenzoic acids had adverse steric effects.
- Observation Substituent effect patterns disagreed with an electrophilic oxidation expectation.
- Observation Substituent effect patterns agreed with expected nucleophilic attack by peroxo anions.
- Mechanism The authors infer Compound I forms through reaction with un-ionized hydroperoxide molecules.