Claim · model-written summary
Predicted molecular mass was similar to that of native GA 20-oxidase.
- Taxon
- pumpkin (Cucurbita maxima L.)
Cucurbita maximae1 - Gene
- GA 20-oxidasee0
- Outcome
- M(r)e0
- Direction
- similare0
- Value
- 43,321e0
- Comparator
- native GA 20-oxidasee0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted M(r) (43,321) and pI (5.3) are similar to those determined experimentally for the native GA 20-oxidase.”
e1“pumpkin (Cucurbita maxima L.)”
Title
T Lange; P Hedden; J E Graebe Proceedings of the National Academy of Sciences 1994. doi:10.1073/pnas.91.18.8552
Other claims from this paper (13)
- Observation A cDNA clone encoding pumpkin GA 20-oxidase was isolated.
- Observation Translation products from cotyledon and endosperm mRNA contained GA12 20-oxidase activity.
- Observation Positive cDNA clones had GA12 20-oxidase activity in single bacteriophage plaques.
- Observation Recombinant protein converted GA12 to GA25 in three steps.
- Observation Recombinant protein converted GA53 to GA17 in three steps.
- Observation Recombinant protein formed GA1 from GA23.
- Observation Recombinant protein formed GA9 from GA24.
- Observation Recombinant protein formed GA20 from GA19.
- Observation The cDNA open reading frame spans 1158 nt.
- Observation The cDNA open reading frame encodes a protein of 386 amino acid residues.
- Association Predicted pI was similar to that of native GA 20-oxidase.
- Observation Derived sequence includes N-terminal and two tryptic peptide sequences of the native enzyme.
- Observation Derived sequence contains regions conserved among non-heme iron dioxygenases.