Claim · model-written summary
Derived sequence contains regions conserved among non-heme iron dioxygenases.
- Taxon
- pumpkin (Cucurbita maxima L.)
Cucurbita maximae2 - Outcome
- regions that are highly conserved in a group of non-heme Fe-containing dioxygenasese0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also contains regions that are highly conserved in a group of non-heme Fe-containing dioxygenases.”
e1“the derived amino acid sequence”
e2“pumpkin (Cucurbita maxima L.)”
Title
T Lange; P Hedden; J E Graebe Proceedings of the National Academy of Sciences 1994. doi:10.1073/pnas.91.18.8552
Other claims from this paper (13)
- Observation A cDNA clone encoding pumpkin GA 20-oxidase was isolated.
- Observation Translation products from cotyledon and endosperm mRNA contained GA12 20-oxidase activity.
- Observation Positive cDNA clones had GA12 20-oxidase activity in single bacteriophage plaques.
- Observation Recombinant protein converted GA12 to GA25 in three steps.
- Observation Recombinant protein converted GA53 to GA17 in three steps.
- Observation Recombinant protein formed GA1 from GA23.
- Observation Recombinant protein formed GA9 from GA24.
- Observation Recombinant protein formed GA20 from GA19.
- Observation The cDNA open reading frame spans 1158 nt.
- Observation The cDNA open reading frame encodes a protein of 386 amino acid residues.
- Association Predicted molecular mass was similar to that of native GA 20-oxidase.
- Association Predicted pI was similar to that of native GA 20-oxidase.
- Observation Derived sequence includes N-terminal and two tryptic peptide sequences of the native enzyme.