Claim · model-written summary
Of 732 regenerated shoot lines from CRISPR/Cas editing of MdDAM1, 60 showed mutations.
- Taxon
- apple
Malus domesticat0 - Outcome
- showed mutationse0
- Value
- 60e0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“Of 732 regenerated shoot lines, 60 showed mutations”
Title
Functional characterization of Dormancy-Associated MADS-Box genes in apple
J. Lempe; S. Schröpfer; L. Baader; et al. Acta Horticulturae 2026. doi:10.17660/actahortic.2026.1454.33
Other claims from this paper (5)
- Observation Several DAM genes were identified on different chromosomes of the apple genome sequence.
- Mechanism Phenotypic and transcriptomic analyses of MdDAM1 and MdDAM4 overexpression or silencing lines confirmed both genes as essential for dormancy regulation.
- Observation Apple trees lacking functional MdDAM1 or MdDAM4 failed to enter dormancy.
- Observation None of the mutated lines had both alleles of MdDAM1 mutated.
- Observation A second regeneration step yielded 17 lines with uniform biallelic MdDAM1 mutations.