Claim · model-written summary
Cryopreserved embryos and axes regenerated normal healthy seedlings and plantlets without intervening callus growth
- Outcome
- normal healthy seedlings from embryos and plantlets from axese5
- Qualifier
- without intervening callus growth in vitroe5
CitrusNot reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five citrus species were cryopreserved”
e5“producing normal healthy seedlings from embryos and plantlets from axes without intervening callus growth in vitro”
Title
S K Malik; R Chaudhury; H W Pritchard PubMed 2013
Other claims from this paper (10)
- Intervention effect Drying to about 10 percent moisture content was about 10 times faster for isolated axes than for embryos
- Observation Optimum moisture contents for cryopreservation were generally similar within a species, from about 10 percent to about 20 percent
- Intervention effect The hydration window for cryopreservation was usually wider for axes than for embryos
- Observation Embryo or axis survival after cryopreservation ranged from 65 to 96 percent across species
- Observation Partially dried embryos of all five species survived 120 days of liquid nitrogen vapour storage
- Observation Viability loss of partially dried embryos was rapid at -20 degree C, 5 degree C and ambient temperature
- Observation The maximum interpolated viability half-life across storage temperatures was about 80 days for C. grandis at 5 degree C
- Method / resource Developed cryopreservation protocols were applied to cryobank 377 citrus germplasm accessions
- Observation Between 69 and 81 percent of accessions per species retained over 70 percent of viability after desiccation following 6.3 to 8.4 years of cryo-storage
- Recommendation The results provide evidence for the importance of cryopreservation for banking seeds of higher plants