Claim · model-written summary
Optimum moisture contents for cryopreservation were generally similar within a species, from about 10 percent to about 20 percent
- Outcome
- optimum MCs for cryopreservatione2
- Value
- c. 10 percent (C. jambhiri) to c. 20 percent (C. medica)e2
- Qualifier
- generally similar within a speciese2
CitrusNot reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five citrus species were cryopreserved”
e2“the optimum MCs for cryopreservation were generally similar within a species, varying from c. 10 percent (C. jambhiri) to c. 20 percent (C. medica)”
Title
S K Malik; R Chaudhury; H W Pritchard PubMed 2013
Other claims from this paper (10)
- Intervention effect Drying to about 10 percent moisture content was about 10 times faster for isolated axes than for embryos
- Intervention effect The hydration window for cryopreservation was usually wider for axes than for embryos
- Observation Embryo or axis survival after cryopreservation ranged from 65 to 96 percent across species
- Observation Cryopreserved embryos and axes regenerated normal healthy seedlings and plantlets without intervening callus growth
- Observation Partially dried embryos of all five species survived 120 days of liquid nitrogen vapour storage
- Observation Viability loss of partially dried embryos was rapid at -20 degree C, 5 degree C and ambient temperature
- Observation The maximum interpolated viability half-life across storage temperatures was about 80 days for C. grandis at 5 degree C
- Method / resource Developed cryopreservation protocols were applied to cryobank 377 citrus germplasm accessions
- Observation Between 69 and 81 percent of accessions per species retained over 70 percent of viability after desiccation following 6.3 to 8.4 years of cryo-storage
- Recommendation The results provide evidence for the importance of cryopreservation for banking seeds of higher plants