Claim · model-written summary
Barley leaf intercellular washing fluid contained glucosidase activity that released ABA from conjugate.
- Taxon
- barley
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Hordeum vulgaree1 - Organ / stage
- leaf apoplaste0
- Gene
- β‐glucosidasee0
- Outcome
- releases abscisic acid (ABA)e0
Not reportedMaterialCompoundInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“IWF contains β‐glucosidase activity which releases abscisic acid (ABA) from the physiologically inactive ABA‐glucose conjugate pool in the leaf apoplast”
e1“barley primary leaves (Hordeum vulgare cv. Gerbel)”
Title
Karl‐Josef Dietz; Angela Sauter; Kathrin Wichert; et al. Journal of Experimental Botany 2000. doi:10.1093/jxb/51.346.937
Other claims from this paper (13)
- Intervention effect Salinity increased ABA conjugate concentration in barley xylem sap.
- Observation ABA conjugate concentration remained low in barley primary-leaf intercellular washing fluid.
- Intervention effect Incubating ABA glucose ester with intercellular washing fluid released free ABA.
- Observation The released product matched authentic ABA on chromatography and ABA-ELISA.
- Observation The barley glucosidase KM for pNPG was 1.8 mmol l−1.
- Intervention effect ABA glucopyranoside competitively inhibited glucosidase activity with KI 400 μmol l−1.
- Intervention effect ABA glucopyranoside showed the highest glucosidase inhibition.
- Intervention effect Zeatin riboside showed the highest glucosidase inhibition.
- Intervention effect ABA methyl ester inhibition of glucosidase was insignificant.
- Intervention effect Zeatin-9-beta-d-glucoside inhibition of glucosidase was insignificant.
- Observation Specific glucosidase activity was 16-fold greater in intercellular washing fluid than crude leaf extracts.
- Intervention effect NaCl-supplemented hydroponic growth strongly increased glucosidase activity.
- Mechanism The data support ABA glucose conjugate as a long-distance ABA transport form.