Claim · model-written summary
Embryos dried to 0.23 g H 2 O g −1 d.wt before freezing germinated at 93.8%.
- Treatment
- 48 h of desiccation at 97% RHe0
- Outcome
- germinatee0
- Direction
- highe0
- Value
- 93.8%e0
- Qualifier
- water content of 0.23 g H 2 O g −1 d.wte0
PiceaNot reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSetting
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“somatic embryos had to reach a water content of 0.23 g H 2 O g −1 d.wt (48 h of desiccation at 97% RH) before immersion in liquid nitrogen to germinate at high frequency (93.8%).”
e1“Picea spp.”
Title
C Bomal Annals of Botany 2000. doi:10.1006/anbo.2000.1176
Other claims from this paper (11)
- Intervention effect Slow drying at 97 or 88% RH produced 96.7–100% germination.
- Intervention effect Slow drying at 97 or 88% RH produced 26.7–46.7% plantlet conversion.
- Intervention effect Thawing technique did not affect embryo survival.
- Observation Dried cryopreserved Picea embryos could reinduce embryogenic tissue.
- Observation Dried cryopreserved Picea embryos could start new embryo production.
- Intervention effect Drying at 97% RH and rehydration at 100% RH for 12 h gave 66.7% reinduction.
- Observation Embryo production did not differ between parent line and sublines.
- Observation Second generation embryos germinated at rates similar to controls.
- Observation Second generation embryos regenerated into plantlets at rates similar to controls.
- Method / resource The optimal cryopreservation method worked across several P. mariana genotypes.
- Method / resource The optimal cryopreservation method worked across several P. glauca genotypes.