Claim · model-written summary
One molar sucrose dehydration gave maximum survival of 50 %.
- Taxon
- date palm
Phoenix dactyliferat0 - Treatment
- 1.0 M sucrosee0
- Outcome
- survivale0
- Direction
- maximume0
- Value
- 50 %e0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maximum values of survival (50 %) and re-growth (45 %) were counted when somatic embryos were dehydrated by 1.0 M sucrose prior to cryopreservation.”
t0“date palm”
Title
Recovery and histological changes of date palm somatic embryos after cryopreservation by dehydration
Shawky Bekheet; Ahmed M. Amer; Valbona Sota Egyptian Journal of Chemistry 2024. doi:10.21608/ejchem.2024.288440.9694
Other claims from this paper (11)
- Intervention effect Air drying for 60 min gave the best somatic embryo survival.
- Intervention effect Air drying for 60 min maximized secondary embryos, germination and proliferated shootlets.
- Intervention effect One molar sucrose dehydration gave maximum regrowth of 45 %.
- Intervention effect One molar sucrose dehydration produced the highest secondary embryo number, 5.90.
- Observation After one molar sucrose dehydration, 85 % of embryos germinated into healthy propagules.
- Intervention effect Dehydration with 1.5 M sucrose produced the highest shootlet number, 5.50.
- Observation Cryopreservation by dehydration caused minimal cellular damage.
- Intervention effect Compared with non-cryopreserved cultures, some cell walls were broken.
- Intervention effect Cryopreservation increased vacuole size and cytoplasm density compared with non-cryopreserved cultures.
- Observation Damaged embryo cells could divide and regenerate into normal tissue.
- Recommendation The authors conclude that osmotic dehydration with 1.0 M sucrose is an effective date palm cryopreservation method.