Claim · model-written summary
Leaf discs inoculated with isolate 433 differentiated resistant, intermediately resistant and susceptible soil-grown leaf material after 10 days.
- Taxon
- Brassica napus subsp. oleifera
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Brassica napus var. napuse0 - Treatment
- Inoculations with isolate 433e7
- Interactor
- Leptosphaeria maculans
Plenodomus lingame0pathogen - Outcome
- differentiation of resistant, intermediately resistant and susceptible leaf materiale7
- Value
- 10 dayse7
- Setting
- soil grown plantse7
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleComparatorDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“winter oilseed rape ( Brassica napus subsp. oleifera ) with resistance to Leptosphaeria maculans”
e7“Inoculations with isolate 433 allowed the differentiation of resistant, intermediately resistant and susceptible leaf material of soil grown plants, when leaf discs from young leaves were incubated on water agar supplemented with BAP (1 × 10 −5 M) at 25°C for 10 days”
Title
Marie A. Gretenkort; D. S. Ingram Journal of Phytopathology 1993. doi:10.1111/j.1439-0434.1993.tb01329.x
Other claims from this paper (12)
- Method / resource Stem inoculation disease scores correlated well (r = 0.92) with resistance ratings of eight of nine soil-grown cultivars.
- Observation The cultivar Liradonna was the exception to the stem inoculation correlation.
- Association Isolates of L. maculans differed in virulence on stems, ranked 478 > 41A4 > 433.
- Association The stem virulence ranking of L. maculans isolates was the inverse of the leaf inoculation ranking.
- Method / resource Stem inoculation of in vitro shoot cultures differentiated resistant and susceptible cultivars after 20 days at 20°C.
- Observation The stem protocol was applicable to plantlets regenerated from thin cell layer explants grown in vitro.
- Observation Intermediately resistant leaves were resistant after 10 days of incubation.
- Observation Intermediately resistant leaves were susceptible after 15 days of incubation.
- Association Leaves of in vitro shoot cultures were more susceptible than the corresponding soil-grown material.
- Method / resource Inoculation of old leaves with isolate 41A4 distinguished the cultivars after 15 days of incubation.
- Observation The protocols allow accurate assessment of resistance to L. maculans at the stem or leaf level.
- Recommendation The protocols are of use in traditional and in vitro oilseed rape selection programmes.