Claim · model-written summary
Leaves of in vitro shoot cultures were more susceptible than the corresponding soil-grown material.
- Taxon
- Brassica napus subsp. oleifera
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Brassica napus var. napuse0 - Organ / stage
- Leaves of shoot culturese1
- Interactor
- Leptosphaeria maculans
Plenodomus lingame0pathogen - Outcome
- susceptiblee1
- Direction
- moree1
- Comparator
- corresponding soil grown materiale1
- Setting
- in vitroe1
Not reportedMaterialGeneCompoundSecond interactorSecond interactor roleTreatmentValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“winter oilseed rape ( Brassica napus subsp. oleifera ) with resistance to Leptosphaeria maculans”
e1“Leaves of shoot cultures grown in vitro were more susceptible than the corresponding soil grown material”
Title
Marie A. Gretenkort; D. S. Ingram Journal of Phytopathology 1993. doi:10.1111/j.1439-0434.1993.tb01329.x
Other claims from this paper (12)
- Method / resource Stem inoculation disease scores correlated well (r = 0.92) with resistance ratings of eight of nine soil-grown cultivars.
- Observation The cultivar Liradonna was the exception to the stem inoculation correlation.
- Association Isolates of L. maculans differed in virulence on stems, ranked 478 > 41A4 > 433.
- Association The stem virulence ranking of L. maculans isolates was the inverse of the leaf inoculation ranking.
- Method / resource Stem inoculation of in vitro shoot cultures differentiated resistant and susceptible cultivars after 20 days at 20°C.
- Observation The stem protocol was applicable to plantlets regenerated from thin cell layer explants grown in vitro.
- Method / resource Leaf discs inoculated with isolate 433 differentiated resistant, intermediately resistant and susceptible soil-grown leaf material after 10 days.
- Observation Intermediately resistant leaves were resistant after 10 days of incubation.
- Observation Intermediately resistant leaves were susceptible after 15 days of incubation.
- Method / resource Inoculation of old leaves with isolate 41A4 distinguished the cultivars after 15 days of incubation.
- Observation The protocols allow accurate assessment of resistance to L. maculans at the stem or leaf level.
- Recommendation The protocols are of use in traditional and in vitro oilseed rape selection programmes.