Claim · model-written summary
Incubation at 32[deg]C inhibited TMV induction of BA2H activity and SA accumulation in inoculated tobacco.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Treatment
- incubated at 32[deg]Ce5
- Interactor
- TMV
Tobacco mosaic viruse5pathogen - Gene
- BA2He5
- Outcome
- induction of BA2H activity and SA accumulatione5
- Direction
- inhibitede5
Not reportedMaterialCompoundOrgan / stageSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Virus-Inoculated Tobacco”
e5“TMV induction of BA2H activity and SA accumulation were inhibited when inoculated tobacco plants were incubated at 32[deg]C”
Title
Induction of Benzoic Acid 2-Hydroxylase in Virus-Inoculated Tobacco
J. Leon; N. Yalpani; I. Raskin; et al. PLANT PHYSIOLOGY 1993. doi:10.1104/pp.103.2.323
Other claims from this paper (13)
- Observation BA2H activity in healthy tobacco leaf extracts was 1-2 nmol h-1 g-1 fresh weight.
- Intervention effect Inoculation with tobacco mosaic virus significantly increased BA2H activity in tobacco leaves compared with healthy leaf extracts.
- Association The increase in BA2H activity after TMV inoculation paralleled the levels of free SA in the leaves.
- Intervention effect Inoculated tobacco plants incubated at 32[deg]C for 4 d and then moved to 24[deg]C showed a 15-fold increase in BA2H activity over healthy plants at 24[deg]C.
- Intervention effect Inoculated tobacco plants incubated at 32[deg]C for 4 d and then moved to 24[deg]C showed a 65-fold increase in free SA content over healthy plants at 24[deg]C.
- Intervention effect Cycloheximide blocked the induction of BA2H activity by TMV in tobacco leaf tissue.
- Intervention effect Infiltrating healthy leaf discs with BA could duplicate the effect of TMV inoculation on BA2H.
- Intervention effect The BA2H response was observed even when applied BA levels were much lower than the levels in vivo after virus inoculation.
- Intervention effect Feeding tobacco leaves with phenylalanine did not trigger BA2H activity induction.
- Intervention effect Feeding tobacco leaves with cinnamic acid did not trigger BA2H activity induction.
- Intervention effect Feeding tobacco leaves with o-coumaric acid did not trigger BA2H activity induction.
- Mechanism BA2H, a pathogen-inducible protein, appears to have a regulatory role in SA accumulation during induced resistance to TMV in tobacco.
- Observation Induction of BA2H activity was restricted to tissue expressing a hypersensitive response at 24[deg]C.