Claim · model-written summary
Actinomycin-D did not nullify the basal incorporation of orotic acid into RNA.
- Taxon
- broccoli
Brassica oleracea var. italicae0 - Treatment
- Actinomycin-De5
- Outcome
- basal incorporation of orotic acid into RNAe5
- Direction
- did not nullifye5
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e5“Actinomycin-D did not nullify the basal incorporation of orotic acid into RNA, nor did it negate the effect of kinetin upon incorporation”
Title
G. J. Vonabrams; Harlan K. Pratt PLANT PHYSIOLOGY 1968. doi:10.1104/pp.43.8.1271
Other claims from this paper (16)
- Intervention effect Kinetin increased net uptake of orotic acid and leucine in detached broccoli leaves.
- Intervention effect Naphthaleneacetic acid decreased the effect of kinetin on net uptake after long treatment.
- Intervention effect In short-time treatments auxin increased the effect of kinetin on net uptake.
- Association Long treatments showed general synchrony between the loss of RNA, protein and chlorophyll.
- Intervention effect Naphthaleneacetic acid reduced the stabilizing effect of kinetin on chlorophyll content.
- Intervention effect Naphthaleneacetic acid reduced the content and synthesis of RNA in the presence of kinetin.
- Intervention effect In short-time experiments kinetin transiently increased RNA content and synthesis.
- Intervention effect Kinetin plus naphthaleneacetic acid further increased RNA content and synthesis compared with kinetin.
- Intervention effect Kinetin decreased chlorophyll content in short-time experiments.
- Intervention effect Kinetin plus naphthaleneacetic acid decreased chlorophyll content further than kinetin alone.
- Intervention effect Actinomycin-D accelerated the loss of chlorophyll, RNA and protein.
- Intervention effect Actinomycin-D strongly depressed the rate of RNA synthesis.
- Intervention effect Actinomycin-D substantially reduced the stabilizing effect of kinetin upon RNA.
- Intervention effect Chlorophyll and protein contents remained higher than in the control with actinomycin-D.
- Intervention effect Actinomycin-D did not negate the effect of kinetin upon incorporation.
- Mechanism The results do not substantiate that kinetin regulates senescence by a direct effect upon DNA-dependent RNA synthesis.