Abstract
The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein. Kinetin increased the net uptake of (14)C-labeled orotic acid and leucine. Naphthaleneacetic acid decreased the effect of kinetin on net uptake after long treatment, but in short-time treatments the auxin increased the effect of kinetin on net uptake. Results of long (24 hr) treatments indicated a general synchrony between the loss of RNA, protein, and chlorophyll. Naphthaleneacetic acid reduced the stabilizing effect of kinetin upon chlorophyll content and upon the content and synthesis of RNA. In short-time experiments, however, RNA content and synthesis were transiently increased by kinetin, and further increased by kinetin plus naphthaleneacetic acid, while chlorophyll content decreased in the presence of kinetin and decreased further in the presence of kinetin plus naphthaleneacetic acid. Actinomycin-D accelerated the loss of chlorophyll, RNA and protein and strongly depressed the rate of RNA synthesis. In the presence of actinomycin-D the stabilizing effect of kinetin upon RNA was substantially reduced. In contrast, the chlorophyll and protein contents remained higher than in the control. Actinomycin-D did not nullify the basal incorporation of orotic acid into RNA, nor did it negate the effect of kinetin upon incorporation. The failure of synchrony between changes in chlorophyll and RNA does not substantiate the proposal that kinetin regulates senescence by a direct effect upon DNA-dependent RNA synthesis.
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Claims 17
Claim · model-written summary
Kinetin increased net uptake of orotic acid and leucine in detached broccoli leaves.
- Taxon
- broccoli
Brassica oleracea var. italicae0 - Treatment
- Kinetine1
- Outcome
- net uptake of (14)C-labeled orotic acid and leucinee1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e1“Kinetin increased the net uptake of (14)C-labeled orotic acid and leucine”
Claim · model-written summary
Naphthaleneacetic acid decreased the effect of kinetin on net uptake after long treatment.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- Naphthaleneacetic acide2
- Outcome
- effect of kinetin on net uptakee2
- Direction
- decreasede2
- Qualifier
- after long treatmente2
- Comparator
- kinetine2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e2“Naphthaleneacetic acid decreased the effect of kinetin on net uptake after long treatment, but in short-time treatments the auxin increased the effect of kinetin on net uptake”
Claim · model-written summary
In short-time treatments auxin increased the effect of kinetin on net uptake.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- auxine2
- Outcome
- effect of kinetin on net uptakee2
- Direction
- increasede2
- Qualifier
- in short-time treatmentse2
- Comparator
- kinetine2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e2“Naphthaleneacetic acid decreased the effect of kinetin on net uptake after long treatment, but in short-time treatments the auxin increased the effect of kinetin on net uptake”
Claim · model-written summary
Long treatments showed general synchrony between the loss of RNA, protein and chlorophyll.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- long (24 hr) treatmentse3
- Outcome
- loss of RNA, protein, and chlorophylle3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e3“Results of long (24 hr) treatments indicated a general synchrony between the loss of RNA, protein, and chlorophyll”
Claim · model-written summary
Naphthaleneacetic acid reduced the stabilizing effect of kinetin on chlorophyll content.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- Naphthaleneacetic acide4
- Outcome
- stabilizing effect of kinetin upon chlorophyll contente4
- Direction
- reducede4
- Comparator
- kinetine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e4“Naphthaleneacetic acid reduced the stabilizing effect of kinetin upon chlorophyll content and upon the content and synthesis of RNA”
Claim · model-written summary
Naphthaleneacetic acid reduced the content and synthesis of RNA in the presence of kinetin.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- Naphthaleneacetic acide4
- Outcome
- the content and synthesis of RNAe4
- Direction
- reducede4
- Comparator
- kinetine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e4“Naphthaleneacetic acid reduced the stabilizing effect of kinetin upon chlorophyll content and upon the content and synthesis of RNA”
Claim · model-written summary
In short-time experiments kinetin transiently increased RNA content and synthesis.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- kinetine5
- Outcome
- RNA content and synthesise5
- Direction
- transiently increasede5
- Qualifier
- In short-time experimentse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e5“In short-time experiments, however, RNA content and synthesis were transiently increased by kinetin, and further increased by kinetin plus naphthaleneacetic acid, while chlorophyll content decreased in the presence of kinetin and decreased further in the presence of kinetin plus naphthaleneacetic acid”
Claim · model-written summary
Kinetin plus naphthaleneacetic acid further increased RNA content and synthesis compared with kinetin.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- kinetin plus naphthaleneacetic acide5
- Outcome
- RNA content and synthesise5
- Direction
- further increasede5
- Comparator
- kinetine5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e5“In short-time experiments, however, RNA content and synthesis were transiently increased by kinetin, and further increased by kinetin plus naphthaleneacetic acid, while chlorophyll content decreased in the presence of kinetin and decreased further in the presence of kinetin plus naphthaleneacetic acid”
Claim · model-written summary
Kinetin decreased chlorophyll content in short-time experiments.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- kinetine5
- Outcome
- chlorophyll contente5
- Direction
- decreasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e5“In short-time experiments, however, RNA content and synthesis were transiently increased by kinetin, and further increased by kinetin plus naphthaleneacetic acid, while chlorophyll content decreased in the presence of kinetin and decreased further in the presence of kinetin plus naphthaleneacetic acid”
Claim · model-written summary
Kinetin plus naphthaleneacetic acid decreased chlorophyll content further than kinetin alone.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- kinetin plus naphthaleneacetic acide5
- Outcome
- chlorophyll contente5
- Direction
- decreased furthere5
- Comparator
- kinetine5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e5“In short-time experiments, however, RNA content and synthesis were transiently increased by kinetin, and further increased by kinetin plus naphthaleneacetic acid, while chlorophyll content decreased in the presence of kinetin and decreased further in the presence of kinetin plus naphthaleneacetic acid”
Claim · model-written summary
Actinomycin-D accelerated the loss of chlorophyll, RNA and protein.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- Actinomycin-De6
- Outcome
- loss of chlorophyll, RNA and proteine6
- Direction
- acceleratede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e6“Actinomycin-D accelerated the loss of chlorophyll, RNA and protein and strongly depressed the rate of RNA synthesis”
Claim · model-written summary
Actinomycin-D strongly depressed the rate of RNA synthesis.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- Actinomycin-De6
- Outcome
- rate of RNA synthesise6
- Direction
- depressede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e6“Actinomycin-D accelerated the loss of chlorophyll, RNA and protein and strongly depressed the rate of RNA synthesis”
Claim · model-written summary
Actinomycin-D substantially reduced the stabilizing effect of kinetin upon RNA.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- actinomycin-De7
- Outcome
- stabilizing effect of kinetin upon RNAe7
- Direction
- reducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e7“In the presence of actinomycin-D the stabilizing effect of kinetin upon RNA was substantially reduced”
Claim · model-written summary
Chlorophyll and protein contents remained higher than in the control with actinomycin-D.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- actinomycin-De7
- Outcome
- chlorophyll and protein contentse4
- Direction
- highere4
- Comparator
- controle4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e7“In the presence of actinomycin-D the stabilizing effect of kinetin upon RNA was substantially reduced”
e4“In contrast, the chlorophyll and protein contents remained higher than in the control”
Claim · model-written summary
Actinomycin-D did not nullify the basal incorporation of orotic acid into RNA.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- Actinomycin-De5
- Outcome
- basal incorporation of orotic acid into RNAe5
- Direction
- did not nullifye5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e5“Actinomycin-D did not nullify the basal incorporation of orotic acid into RNA, nor did it negate the effect of kinetin upon incorporation”
Claim · model-written summary
Actinomycin-D did not negate the effect of kinetin upon incorporation.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Treatment
- Actinomycin-De5
- Outcome
- effect of kinetin upon incorporatione5
- Direction
- negatee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e5“Actinomycin-D did not nullify the basal incorporation of orotic acid into RNA, nor did it negate the effect of kinetin upon incorporation”
Claim · model-written summary
The results do not substantiate that kinetin regulates senescence by a direct effect upon DNA-dependent RNA synthesis.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Outcome
- kinetin regulates senescence by a direct effecte6
- Qualifier
- upon DNA-dependent RNA synthesise6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein”
e6“The failure of synchrony between changes in chlorophyll and RNA does not substantiate the proposal that kinetin regulates senescence by a direct effect upon DNA-dependent RNA synthesis”
The same questions in other papers
1-naphthaleneacetic acid · molecular: metabolites · Brassica oleracea (wild cabbage)Positive and negative results
1 in the opposite direction
- negative
1-naphthaleneacetic acid · physiology: pigments · Brassica oleracea (wild cabbage)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- positive
- positive
kinetin · physiology: pigments · Brassica oleracea (wild cabbage)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
kinetin · molecular: metabolites · Brassica oleracea (wild cabbage)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.