Claim · model-written summary
Synthetic nodal explants stored at 18ºC kept 80% germination ability.
- Taxon
- Enicostema axillare
Enicostema axillaret0 - Organ / stage
- nodal explantse5
- Treatment
- stored at 18ºCe5
- Outcome
- germination abilitye5
- Direction
- without losinge5
- Value
- 80%e5
Not reportedMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Synthetic Seed Production and Germplasm Conservation of Enicostema axillare (Lam.) raynal”
e5“The synseed nodal explants prepared using ¼ strength MS medium and stored at 18ºC were preserved for up to 80 days and more without losing germination ability (80%)”
Title
Kousalya Loganathan; Packiaraj Sankaran; Sankarlingam Subbiah; et al. Current Applied Science and Technology 2022. doi:10.55003/cast.2022.06.22.001
Other claims from this paper (12)
- Method / resource Synthetic seed prepared with 3% sodium alginate and 100 mM calcium chloride was the optimum for uniform and compact synseed formation.
- Intervention effect Synthetic seeds from full strength MS medium gave the highest number of multiple shoots on BAP 2 mg/l and KIN 0.5 mg/l with GA3.
- Intervention effect Encapsulated nodal explants produced 15.0±1.87 multiple shoots.
- Intervention effect Encapsulated nodal explants germinated at a high rate of 87%.
- Intervention effect Encapsulated nodal explants reached a shoot length of 6.24 ±1.03 cm.
- Intervention effect Encapsulated nodal explants produced a root number of 28.4±5.50.
- Intervention effect Storage at 18ºC was favorable for preservation of synthetic seeds.
- Intervention effect Storage at 18ºC increased synthetic seed germination frequency.
- Intervention effect Synthetic nodal explants stored at 18ºC were preserved for up to 80 days.
- Intervention effect Fully developed plantlets were hardened on vermiculite at 100%.
- Intervention effect Hardened plantlets on vermiculite had a survival rate of 71%.
- Recommendation The protocol is recommended for germplasm conservation of Enicostema axillare by long-term storage.