Claim · model-written summary
XynA and XynB were active when expressed in tobacco, with temperature optima at 85 degrees C.
- Taxon
- tobacco
Nicotiana tabacume0 - Gene
- both enzymese0
- Outcome
- expressed in an active forme0
- Value
- 85 degrees Ce0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression in tobacco and stable expression in transgenic Arabidopsis showed that both enzymes were expressed in an active form with temperature optima at 85 degrees C.”
Title
Autohydrolysis of plant xylans by apoplastic expression of thermophilic bacterial endo‐xylanases
Bernhard Borkhardt; Jesper Harholt; Peter Ulvskov; et al. Plant Biotechnology Journal 2010. doi:10.1111/j.1467-7652.2010.00506.x
Other claims from this paper (6)
- Observation XynA and XynB were active when expressed in transgenic Arabidopsis, with temperature optima at 85 degrees C.
- Observation Transgenic Arabidopsis accumulating endo-xylanases appeared phenotypically normal.
- Observation Transgenic Arabidopsis accumulating endo-xylanases was fully fertile.
- Observation Arabidopsis dry stems had the highest xylanase activity.
- Observation Xylanase activity persisted in dry transgenic Arabidopsis stem extracts for 24 hours at 85 degrees C.
- Intervention effect Heat-treated transgenic stem extracts had reduced xylan molecular weight compared with wild type.