Abstract
The genes encoding the two endo-xylanases XynA and XynB from the thermophilic bacterium Dictyoglomus thermophilum were codon optimized for expression in plants. Both xylanases were designed to be constitutively expressed under the control of the CaMV 35S promoter and targeted to the apoplast. Transient expression in tobacco and stable expression in transgenic Arabidopsis showed that both enzymes were expressed in an active form with temperature optima at 85 degrees C. Transgenic Arabidopsis accumulating heterologous endo-xylanases appeared phenotypically normal and were fully fertile. The highest xylanase activity in Arabidopsis was found in dry stems indicating that the enzymes were not degraded during stem senescence. High levels of enzyme activity were maintained in cell-free extracts from dry transgenic stems during incubation at 85 degrees C for 24 h. Analysis of cell wall polysaccharides after heat treatment of wildtype and transgenic extracts from dry stems showed a decrease in the molecular weight of xylans from transgenic stems.
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Claims 7
Claim · model-written summary
XynA and XynB were active when expressed in tobacco, with temperature optima at 85 degrees C.
- Taxon
- tobacco
Nicotiana tabacume0 - Gene
- both enzymese0
- Outcome
- expressed in an active forme0
- Value
- 85 degrees Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression in tobacco and stable expression in transgenic Arabidopsis showed that both enzymes were expressed in an active form with temperature optima at 85 degrees C.”
Claim · model-written summary
XynA and XynB were active when expressed in transgenic Arabidopsis, with temperature optima at 85 degrees C.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- transgenic Arabidopsise0
- Gene
- both enzymese0
- Outcome
- expressed in an active forme0
- Value
- 85 degrees Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression in tobacco and stable expression in transgenic Arabidopsis showed that both enzymes were expressed in an active form with temperature optima at 85 degrees C.”
Claim · model-written summary
Transgenic Arabidopsis accumulating endo-xylanases appeared phenotypically normal.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Transgenic Arabidopsise0
- Gene
- heterologous endo-xylanasese0
- Outcome
- phenotypically normale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic Arabidopsis accumulating heterologous endo-xylanases appeared phenotypically normal and were fully fertile.”
Claim · model-written summary
Transgenic Arabidopsis accumulating endo-xylanases was fully fertile.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Transgenic Arabidopsise0
- Gene
- heterologous endo-xylanasese0
- Outcome
- fully fertilee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic Arabidopsis accumulating heterologous endo-xylanases appeared phenotypically normal and were fully fertile.”
Claim · model-written summary
Arabidopsis dry stems had the highest xylanase activity.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- dry stemse0
- Gene
- xylanasee0
- Outcome
- highest xylanase activitye0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest xylanase activity in Arabidopsis was found in dry stems indicating that the enzymes were not degraded during stem senescence.”
Claim · model-written summary
Xylanase activity persisted in dry transgenic Arabidopsis stem extracts for 24 hours at 85 degrees C.
Verbatim
e0“High levels of enzyme activity were maintained in cell-free extracts from dry transgenic stems during incubation at 85 degrees C for 24 h.”
t0“plant xylans”
Claim · model-written summary
Heat-treated transgenic stem extracts had reduced xylan molecular weight compared with wild type.
Verbatim
e0“Analysis of cell wall polysaccharides after heat treatment of wildtype and transgenic extracts from dry stems showed a decrease in the molecular weight of xylans from transgenic stems.”
t0“plant xylans”