Claim · model-written summary
Feeding labeled glucose to sdp6 seedlings showed a sixfold reduction in label transfer to the opposing sucrose hexosyl moiety.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- sdp6e0
- Organ / stage
- seedlingse0
- Treatment
- [U-(14)C]glucosee0
- Outcome
- transfer of (14)C-label into the opposing hexosyl moiety of sucrosee0
- Direction
- reductione0
- Value
- 6-folde0
Not reportedGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there is a 6-fold reduction in the transfer of (14)C-label into the opposing hexosyl moiety of sucrose when [U-(14)C]glucose or [U-(14)C]fructose is fed to sdp6 seedlings”
t0“Arabidopsis”
Title
Anne-Laure Quettier; Eve Shaw; Peter J. Eastmond PLANT PHYSIOLOGY 2008. doi:10.1104/pp.108.123703
Other claims from this paper (16)
- Observation sdp6 mutants could break down triacylglycerol after germination.
- Observation sdp6 mutants failed to accumulate soluble sugars after germination.
- Mechanism sdp6 mutant results suggest a gluconeogenesis defect.
- Observation SDP6 encodes mitochondrial FAD-GPDH.
- Observation Germinated sdp6 seeds were severely impaired in glycerol metabolism to carbon dioxide.
- Observation Germinated sdp6 seeds accumulated high G3P levels.
- Mechanism The data suggest SDP6 is essential for glycerol catabolism.
- Intervention effect G3P competitively inhibited phosphoglucose isomerase activity in vitro.
- Observation Feeding labeled fructose to sdp6 seedlings showed a sixfold reduction in label transfer to the opposing sucrose hexosyl moiety.
- Mechanism Phosphoglucose isomerase is likely inhibited in vivo in sdp6 seedlings.
- Mechanism A gluconeogenesis block at hexose phosphate isomerization could explain arrested sdp6 seedling growth.
- Intervention effect Sucrose rescues the arrested sdp6 seedling growth phenotype.
- Intervention effect Glucose rescues the arrested sdp6 seedling growth phenotype.
- Intervention effect Fructose does not rescue the arrested sdp6 seedling growth phenotype.
- Observation NAD(+) and NADH measurements suggest altered NAD(+)/NADH homeostasis in sdp6 seedlings.
- Mechanism The homeostasis result supports the hypothesis that SDP6 cooperates with GPDHC1 in a mitochondrial G3P shuttle.