Claim · model-written summary
Site-specific mutagenesis of the PKA phosphorylation site did not affect localization of MoGti1 to the nucleus.
- Taxon
- cultivated rice
Oryza sativae0 - Treatment
- Site-specific mutagenesise4
- Gene
- MoGti1e4
- Outcome
- localization of MoGti1 to the nucleuse4
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hemibiotrophic pathogen Magnaporthe oryzae causes one of the most destructive diseases in cultivated rice”
e4“Site-specific mutagenesis analyses showed that the putative protein kinase A phosphorylation site was not essential for localization of MoGti1 to the nucleus but important for its normal function. Although the cyclin-dependent kinase (CDK) phosphorylation site of MoGti1 is dispensable during vegetative growth and appressorium formation, the S77A mutation affected penetration and invasive growth. Localization of MoGti1(S77A)-green fluorescent protein to the nucleus in late stages of appressorium formation and during invasive growth was not observed”
Title
Yang Li; Guanghui Wang; Jin-Rong Xu; et al. Molecular Plant-Microbe Interactions 2015. doi:10.1094/mpmi-06-15-0142-r
Other claims from this paper (14)
- Intervention effect The Mogti1 mutant of Magnaporthe oryzae was nonpathogenic on rice.
- Intervention effect The Mogti1 mutant had normal appressorium formation and turgor generation.
- Intervention effect The Mogti1 mutant was defective in penetration and in growth of normal invasive hyphae on rice.
- Intervention effect Deletion of MoGTI1 affected the expression of most effector genes of Magnaporthe oryzae.
- Mechanism MoGti1 expression appears to be controlled by Mps1 but not by Pmk1 MAPK.
- Intervention effect The mps1 and Mogti1 mutants had similar phenotypes in plant infection and cell wall integrity on rice.
- Mechanism MoGti1 is probably not a direct target of Mps1.
- Intervention effect The PKA phosphorylation site of MoGti1 is important for its normal function.
- Intervention effect The S77A mutation of MoGti1 affected penetration and invasive growth on rice.
- Intervention effect The CDK phosphorylation site of MoGti1 is dispensable for vegetative growth and appressorium formation.
- Intervention effect Localization of MoGti1(S77A)-GFP to the nucleus in late stages of appressorium formation and during invasive growth was not observed.
- Mechanism Mps1 may indirectly regulate MoGti1 expression in maintaining cell wall integrity, conidiation and plant infection.
- Mechanism MoGti1 is likely a stage-specific target of CDK.
- Mechanism MoGti1 plays a crucial role in effector gene expression and morphogenesis of penetration pegs and invasive hyphae.