Claim · model-written summary
NbRae1 depletion in the shoot apex decreased expression of histones H3 and H4.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Organ / stage
- shoot apexe1
- Treatment
- depletion of NbRae1e1
- Gene
- histones H3 and H4e2
- Outcome
- expressione2
- Direction
- decreasede2
Not reportedMaterialCompoundInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus-induced gene silencing of Nicotiana benthamiana Rae1, NbRae1”
e1“In the shoot apex, depletion of NbRae1 led to reduced mitotic activities”
e2“decreased expression of cyclin B1, CDKB1-1, and histones H3 and H4”
Title
Dual functions of Nicotiana benthamiana Rae1 in interphase and mitosis
Jae‐Yong Lee; Ho‐Seok Lee; Soo‐Jin Wi; et al. The Plant Journal 2009. doi:10.1111/j.1365-313x.2009.03869.x
Other claims from this paper (13)
- Observation Virus-induced silencing of NbRae1 resulted in growth arrest and abnormal leaf development.
- Observation NbRae1 was mainly associated with the nuclear envelope during interphase.
- Intervention effect NbRae1 deficiency caused accumulation of poly(A) RNA in the nuclei of leaf cells, suggesting defective mRNA export.
- Intervention effect In the shoot apex, depletion of NbRae1 reduced mitotic activities.
- Intervention effect NbRae1 depletion in the shoot apex reduced cyclin-dependent kinase activity.
- Intervention effect NbRae1 depletion in the shoot apex decreased expression of cyclin B1 and CDKB1-1.
- Intervention effect Silencing of NbRae1 inhibited secondary growth of stem vasculature, indicating reduced cambial activity.
- Intervention effect Differentiated leaf cells of NbRae1-silenced plants exhibited elevated ploidy levels.
- Observation NbRae1 protein localized to mitotic microtubules and the cell plate-forming zone during mitosis.
- Observation Recombinant NbRae1 directly bound to microtubules in vitro.
- Observation Inhibition of NbRae1 expression in BY-2 cells with an inducible RNAi system caused severe defects in spindle organization and chromosome alignment and segregation.
- Observation Inhibition of NbRae1 expression correlated with delays in cell cycle progression.
- Mechanism NbRae1 plays a dual role in mRNA export in interphase and in spindle assembly in mitosis.