Claim · model-written summary
Silencing of NbRae1 inhibited secondary growth of stem vasculature, indicating reduced cambial activity.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Organ / stage
- stem vasculaturee1
- Treatment
- Virus-induced gene silencinge0
- Outcome
- secondary growthe1
- Direction
- inhibitede1
- Qualifier
- indicating reduced cambial activitiese1
Not reportedMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus-induced gene silencing of Nicotiana benthamiana Rae1, NbRae1”
e1“The secondary growth of stem vasculature was also inhibited, indicating reduced cambial activities”
Title
Dual functions of Nicotiana benthamiana Rae1 in interphase and mitosis
Jae‐Yong Lee; Ho‐Seok Lee; Soo‐Jin Wi; et al. The Plant Journal 2009. doi:10.1111/j.1365-313x.2009.03869.x
Other claims from this paper (13)
- Observation Virus-induced silencing of NbRae1 resulted in growth arrest and abnormal leaf development.
- Observation NbRae1 was mainly associated with the nuclear envelope during interphase.
- Intervention effect NbRae1 deficiency caused accumulation of poly(A) RNA in the nuclei of leaf cells, suggesting defective mRNA export.
- Intervention effect In the shoot apex, depletion of NbRae1 reduced mitotic activities.
- Intervention effect NbRae1 depletion in the shoot apex reduced cyclin-dependent kinase activity.
- Intervention effect NbRae1 depletion in the shoot apex decreased expression of cyclin B1 and CDKB1-1.
- Intervention effect NbRae1 depletion in the shoot apex decreased expression of histones H3 and H4.
- Intervention effect Differentiated leaf cells of NbRae1-silenced plants exhibited elevated ploidy levels.
- Observation NbRae1 protein localized to mitotic microtubules and the cell plate-forming zone during mitosis.
- Observation Recombinant NbRae1 directly bound to microtubules in vitro.
- Observation Inhibition of NbRae1 expression in BY-2 cells with an inducible RNAi system caused severe defects in spindle organization and chromosome alignment and segregation.
- Observation Inhibition of NbRae1 expression correlated with delays in cell cycle progression.
- Mechanism NbRae1 plays a dual role in mRNA export in interphase and in spindle assembly in mitosis.