Abstract
Plant pathogens secrete effector molecules that suppress the plant immune response to facilitate disease development. AvrPto is a well-studied effector from the phytopathogenic bacterium Pseudomonas syringae. Here we utilise an in planta proximity dependent biotin ligase labelling technique (BioID) in combination with AvrPto to identify proximal proteins that are potential immune system components. The labelling technique biotinylated proteins proximal to AvrPto at the plasma-membrane allowing their isolation and identification by mass spectrometry. Five AvrPto proximal plant proteins (APPs) were identified and their effect on plant immune function and growth was examined in Nicotiana benthamiana leaves. One protein identified, RIN4, is a central immune component previously shown to interact with AvrPto. Two other proteins were identified which form a complex and when silenced significantly reduced Pseudomonas syringe tabaci growth. The first was a receptor like protein kinase (APK1) which was required for Pto/Prf signalling and the second was Target of Myb1 (TOM1), a membrane associated protein with a phosphatidylinositol 5-phosphate (PtdIns5P) binding motif. We have developed a technology to rapidly determine protein interactions within living plant tissue. It is particularly useful for identifying plant immune system components by defining pathogenic effector protein interactions within their plant hosts.
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Claims 6
Claim · model-written summary
BioID labelled proteins near AvrPto at the plasma membrane for mass spectrometry identification.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Outcome
- biotinylated proteins proximal to AvrPtoe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The labelling technique biotinylated proteins proximal to AvrPto at the plasma-membrane allowing their isolation and identification by mass spectrometry”
e1“Nicotiana benthamiana leaves”
Claim · model-written summary
Five plant proteins proximal to AvrPto were identified.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Outcome
- AvrPto proximal plant proteinse0
- Value
- Fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five AvrPto proximal plant proteins (APPs) were identified”
e1“Nicotiana benthamiana leaves”
Claim · model-written summary
RIN4 was identified among the AvrPto proximal proteins.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- RIN4e0
- Outcome
- One protein identified, RIN4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One protein identified, RIN4, is a central immune component previously shown to interact with AvrPto”
e1“Nicotiana benthamiana leaves”
Claim · model-written summary
Silencing two proteins that form a complex significantly reduced Pseudomonas syringe tabaci growth.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- silencede0
- Interactor
- Pseudomonas syringe tabacie0pathogen
- Outcome
- Pseudomonas syringe tabaci growthe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two other proteins were identified which form a complex and when silenced significantly reduced Pseudomonas syringe tabaci growth”
e1“Nicotiana benthamiana leaves”
Claim · model-written summary
APK1 was required for Pto/Prf signalling.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- APK1e0
- Outcome
- Pto/Prf signallinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a receptor like protein kinase (APK1) which was required for Pto/Prf signalling”
e1“Nicotiana benthamiana leaves”
Claim · model-written summary
TOM1 was identified as a membrane protein with a PtdIns5P binding motif.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- Target of Myb1 (TOM1)e0
- Outcome
- a membrane associated protein with a phosphatidylinositol 5-phosphate (PtdIns5P) binding motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Target of Myb1 (TOM1), a membrane associated protein with a phosphatidylinositol 5-phosphate (PtdIns5P) binding motif”
e1“Nicotiana benthamiana leaves”
The same questions in other papers
loss of function · growth · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 17 in the opposite direction
- negative
- negative
- no effect
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.