Abstract
Retinoblastoma protein (Rb) plays a key role in cell cycle control, cell differentiation, and apoptosis in animals. In this study, we used virus-induced gene silencing (VIGS) to investigate the cellular functions of Rb in higher plants. VIGS of NbRBR1, which encodes the Nicotiana benthamiana Rb homolog, resulted in growth retardation and abnormal organ development. At the cellular level, Rb suppression caused prolonged cell proliferation in tissues that are normally differentiated, which indicates that Rb is a negative regulator of plant cell division. Furthermore, differentiation of the epidermal pavement cells and trichomes was partially retarded, and stomatal clusters formed in the epidermis, likely due to uncontrolled cell division of stomata precursor cells. Rb suppression also caused extra DNA replication in endoreduplicating leaf cells, suggesting a role of Rb in the endocycle. These Rb phenotypes were accompanied by stimulated transcription of E2F and E2F-regulated S-phase genes. Thus, disruption of Rb function in plants leads to ectopic cell division in major organs that correlates with a delay in cell differentiation as well as increased endoreduplication, which indicates that Rb coordinates these processes in plant organ development.
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Claims 6
Claim · model-written summary
NbRBR1 silencing caused growth retardation and abnormal organ development.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- VIGS of NbRBR1e0
- Gene
- NbRBR1e0
- Outcome
- growth retardation and abnormal organ developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VIGS of NbRBR1, which encodes the Nicotiana benthamiana Rb homolog, resulted in growth retardation and abnormal organ development.”
Claim · model-written summary
Rb suppression prolonged proliferation in normally differentiated tissues.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Rb suppressione0
- Gene
- Rbe0
- Outcome
- cell proliferatione0
- Direction
- prolongede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rb suppression caused prolonged cell proliferation in tissues that are normally differentiated”
e1“Nicotiana benthamiana Rb homolog”
Claim · model-written summary
Rb suppression partially retarded differentiation of pavement cells and trichomes.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Rb suppressione2
- Outcome
- differentiation of the epidermal pavement cells and trichomese0
- Direction
- partially retardede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differentiation of the epidermal pavement cells and trichomes was partially retarded”
e1“Nicotiana benthamiana Rb homolog”
e2“Rb suppression caused prolonged cell proliferation in tissues that are normally differentiated”
Claim · model-written summary
Stomatal clusters formed after Rb suppression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Rb suppressione2
- Outcome
- stomatal clusters formede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stomatal clusters formed in the epidermis”
e1“Nicotiana benthamiana Rb homolog”
e2“Rb suppression caused prolonged cell proliferation in tissues that are normally differentiated”
Claim · model-written summary
Rb suppression caused extra DNA replication in endoreduplicating leaf cells.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Organ / stage
- leaf cellse0
- Treatment
- Rb suppressione0
- Outcome
- DNA replicatione0
- Direction
- extrae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rb suppression also caused extra DNA replication in endoreduplicating leaf cells”
e1“Nicotiana benthamiana Rb homolog”
Claim · model-written summary
Rb phenotypes accompanied stimulated E2F and E2F-regulated S-phase gene transcription.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- E2F and E2F-regulated S-phase genese0
- Outcome
- transcriptione0
- Direction
- stimulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These Rb phenotypes were accompanied by stimulated transcription of E2F and E2F-regulated S-phase genes.”
e1“Nicotiana benthamiana Rb homolog”
The same questions in other papers
loss of function · growth · Nicotiana benthamianaPositive and negative results
16 papers in the same direction as this paper · 4 in the opposite direction
- negative
- positive
- positive
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.