Abstract
Root parasitic weed Phelipanche aegyptiaca is an obligate plant parasite that causes severe damage to host crops. Agriculture crops mainly belong to the Brassicaceae, Leguminosae, Cruciferae, and Solanaceae plant families affected by this parasitic weed, leading to the devastating loss of crop yield and economic growth. This root-specific parasitic plant is not able to complete its life cycle without a suitable host and is dependent on the host plant for nutrient uptake and germination. Therefore, selected parasitic genes of P. aegyptiaca which were known to be upregulated upon interaction with the host were chosen. These genes are essential for parasitism, and reduced activity of these genes could affect host-parasitic interaction and provide resistance to the host against these parasitic weeds. To check and examine the role of these parasitic genes which can affect the development of host resistance, we silenced selected genes in the P. aegyptiaca using the tobacco rattle virus (TRV) based virus-induced gene silencing (VIGS) method. Our results demonstrated that the total number of P. aegyptiaca parasite tubercles attached to the root of the host plant Nicotiana benthamiana was substantially decreased in all the silenced plants. However, silencing of the P. aegyptiaca MNT1 gene which encodes the mannitol transporter showed a significantly reduced number of germinated shoots and tubercles. Thus, our study indicates that the mannitol transport gene of P. aegyptiaca plays a crucial role in parasitic germination, and silencing of the PaMNT1 gene abolishes the germination of parasites on the host roots.
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Claims 4
Claim · model-written summary
Silencing selected parasite genes substantially decreased tubercles attached to Nicotiana roots.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- silenced selected genese1
- Interactor
- P. aegyptiaca
Phelipanche aegyptiacae0parasite - Outcome
- P. aegyptiaca parasite tubercles attached to the roote0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the total number of P. aegyptiaca parasite tubercles attached to the root of the host plant Nicotiana benthamiana was substantially decreased in all the silenced plants”
e1“we silenced selected genes in the P. aegyptiaca”
Claim · model-written summary
Silencing parasite MNT1 significantly reduced germinated shoots.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- silencing of the P. aegyptiaca MNT1 genee0
- Interactor
- P. aegyptiacaPhelipanche aegyptiacae0parasite
- Gene
- P. aegyptiaca MNT1e0
- Outcome
- number of germinated shootse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of the P. aegyptiaca MNT1 gene which encodes the mannitol transporter showed a significantly reduced number of germinated shoots and tubercles”
e1“host plant Nicotiana benthamiana”
Claim · model-written summary
Silencing parasite MNT1 significantly reduced tubercles.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- silencing of the P. aegyptiaca MNT1 genee0
- Interactor
- P. aegyptiacaPhelipanche aegyptiacae0parasite
- Gene
- P. aegyptiaca MNT1e0
- Outcome
- tuberclese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of the P. aegyptiaca MNT1 gene which encodes the mannitol transporter showed a significantly reduced number of germinated shoots and tubercles”
e1“host plant Nicotiana benthamiana”
Claim · model-written summary
The authors infer that parasite mannitol transport is crucial for germination on host roots.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- P. aegyptiacaPhelipanche aegyptiacae0parasite
- Gene
- mannitol transport gene of P. aegyptiacae0
- Outcome
- parasitic germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mannitol transport gene of P. aegyptiaca plays a crucial role in parasitic germination”
e1“host plant Nicotiana benthamiana”
The same questions in other papers
loss of function · growth · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 17 in the opposite direction
- negative
- negative
- no effect
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.