Claim · model-written summary
Species-specific PCR produced products of 548 bp and 519 bp in the three symptomatic root and two infested soil samples, confirming the presence of P. brassicae.
- Taxon
- canola
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Brassica napus var. napuse0 - Interactor
- P. brassicae
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Plasmodiophora brassicaee1pathogen - Outcome
- PCR productse1
- Value
- 548 bp (TC1F/R) and 519 bp (TC2F/R)e1
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“canola (Brassica napus L.)”
e1“PCR products of 548 bp (TC1F/R) and 519 bp (TC2F/R) were produced in the three symptomatic and two infested soil samples, confirming the presence of P. brassicae”
Title
First Report of Clubroot on Canola Caused by Plasmodiophora brassicae in North Dakota
K. Chittem; S. M. Mansouripour; L. E. del Río Mendoza Plant Disease 2014. doi:10.1094/pdis-04-14-0430-pdn
Other claims from this paper (8)
- Observation This is the first report of clubroot on canola in North Dakota and the United States.
- Observation Numerous spots within the affected canola patch had plant mortalities above 80%.
- Observation Dead canola plants had roots with severe galling and clubbing.
- Observation Round resting structures 2.2 to 4.2 um in diameter, resembling those of Plasmodiophora brassicae, were observed in symptomatic root tissues.
- Observation PCR amplicons were not detected in healthy root and soil samples.
- Intervention effect In the greenhouse pathogenicity test, all plants in inoculated pots developed galls four weeks after planting, while plants in control pots were symptomless.
- Observation P. brassicae resting spores were confirmed in the newly developed galls by microscopy and PCR.
- Recommendation The authors recommend that measures be initiated to limit the spread of clubroot before it threatens United States canola production.