Claim · model-written summary
Adding calcium to membranes plus soluble fraction from unstressed roots elevated ATPase activities to the levels seen with the soluble fraction from NaCl-stressed roots.
- Taxon
- Cucumis sativus
Cucumis sativust0 - Treatment
- calciume6
- Outcome
- ATPase activitiese6
- Direction
- elevatede6
- Comparator
- the soluble fraction isolated from NaCl-stressed rootse6
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Inclusion of calcium in the mixture of membranes and the soluble fraction from unstressed roots elevated the ATPase activities to the levels determined with the soluble fraction isolated from NaCl-stressed roots”
t0“Cucumis sativus roots”
Title
Grażyna Kłobus; Małgorzata Janicka‐Russak Physiologia Plantarum 2004. doi:10.1111/j.0031-9317.2004.00306.x
Other claims from this paper (10)
- Intervention effect Salt treatment of cucumber seedlings increased membrane-bound ATPase activity of both the plasma membrane and the tonoplast.
- Observation In both plasma membrane and tonoplast, stimulation of ATP hydrolysis by salt was much higher than stimulation of H+ transport.
- Mechanism The authors suggest salt treatment partially uncoupled the membrane proton pumps in cucumber plants.
- Intervention effect The soluble fraction from unstressed or NaCl-stressed roots stimulated ATPase activities in membranes isolated from unstressed plants.
- Intervention effect The stimulatory effect of the soluble fraction on proton pump activities was considerably enhanced in salt conditions.
- Intervention effect Staurosporine, a protein kinase inhibitor, totally abolished the stimulatory action of the soluble fractions on the membrane proton pumps.
- Intervention effect Okadaic acid, a phosphatase inhibitor, had no effect on the stimulatory action of the soluble fractions on the membrane proton pumps.
- Intervention effect The cation chelator EGTA cancelled the stimulatory effect of calcium ions.
- Intervention effect The calmodulin antagonist W7 cancelled the stimulatory effect of calcium ions.
- Mechanism The authors strongly suggest specific calcium-calmodulin-dependent protein kinases are involved in activation of membrane ATPases under salt stress.