Claim · model-written summary
Dithiothreitol increased maize sperm cell numbers.
Zea maysNot reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“dithiothreitol increased the cell numbers but lowered their viability”
t0“Isolated from Pollen of Zea mays L.”
Title
Improvement of Longevity and Viability of Sperm Cells Isolated from Pollen of Zea mays L.
Guichang Zhang; Connie M. Williams; Mary K. Campenot; et al. PLANT PHYSIOLOGY 1992. doi:10.1104/pp.100.1.47
Other claims from this paper (11)
- Intervention effect A 0.55 m galactose isolation solution increased maize sperm yield 2.5-fold compared with sucrose.
- Intervention effect Suspension of maize sperm cells in galactose solution gave the best longevity among six sugars.
- Intervention effect Buffering the galactose solution with 2 mM MES significantly improved maize sperm longevity.
- Intervention effect Other buffers had no effect or decreased maize sperm longevity and/or viability.
- Intervention effect pH 6.7 was optimal among five tested pH values for maintaining maize sperm longevity and viability.
- Intervention effect Cysteine caused a rapid decrease in maize sperm cell viability.
- Intervention effect Cysteine increased maize sperm cell lysis.
- Intervention effect Dithiothreitol lowered maize sperm cell viability.
- Intervention effect Addition of 0.1% bovine serum albumin increased maize sperm cell numbers and viability.
- Observation About 70% of maize sperm cells remained viable after 72 h of suspension.
- Intervention effect Maize sperm longevity and viability improved in 0.44 m sucrose when the solution was conditioned with MES and bovine serum albumin.