Abstract
Our previous studies showed that the common maize (Zea mays L.) sperm isolation medium (Brewbaker and Kwack salts in 0.44 m sucrose without buffering) caused cell lysis in vitro. In an attempt to remedy this situation, 6 sugars, 10 buffers, 5 pH values, and 3 membrane protective agents were screened to improve longevity and viability of isolated Zea mays sperm cells as estimated by hemacytometry and flow cytometry. Use of 0.55 m galactose in the isolation solution increased sperm yield by 2.5-fold compared with sucrose, and suspension of isolated sperm cells in the galactose solution gave the best longevity among the six sugars. Buffering the galactose solution with 2 mm 2-(N-morpholino)ethanesulfonic acid significantly improved longevity, whereas other buffers had no effect or decreased the longevity and/or viability. Among the five pH values tested (5.0, 6.0, 6.7, 7.0, and 8.0), pH 6.7 appeared to be optimal for maintenance of both longevity and viability. Screening of membrane protectants showed that cysteine caused a rapid decrease in cell viability and increased lysis, whereas dithiothreitol increased the cell numbers but lowered their viability. Addition of 0.1% bovine serum albumin increased cell numbers and viability, and about 70% of the cells remained viable after 72 h of suspension. Cell longevity and viability were also improved in 0.44 m sucrose when the solution was conditioned with 2-(N-morpholino)ethanesulfonic acid and bovine serum albumin. Use of 2-(N-morpholino)ethanesulfonic acid and bovine serum albumin inthe isolation and suspension medium significantly improved the viability and longevity of sperm cells isolated from Zea mays pollen.
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Claims 12
Claim · model-written summary
A 0.55 m galactose isolation solution increased maize sperm yield 2.5-fold compared with sucrose.
- Treatment
- 0.55 m galactose in the isolation solutione1
- Outcome
- sperm yielde1
- Direction
- increasede1
- Value
- 2.5-folde1
- Comparator
- sucrosee1
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Use of 0.55 m galactose in the isolation solution increased sperm yield by 2.5-fold compared with sucrose”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
Suspension of maize sperm cells in galactose solution gave the best longevity among six sugars.
- Treatment
- suspension of isolated sperm cells in the galactose solutione2
- Outcome
- longevitye2
- Direction
- beste2
- Comparator
- the six sugarse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“suspension of isolated sperm cells in the galactose solution gave the best longevity among the six sugars”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
Buffering the galactose solution with 2 mM MES significantly improved maize sperm longevity.
- Treatment
- Buffering the galactose solution with 2 mm 2-(N-morpholino)ethanesulfonic acide3
- Outcome
- longevitye3
- Direction
- improvede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Buffering the galactose solution with 2 mm 2-(N-morpholino)ethanesulfonic acid significantly improved longevity”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
Other buffers had no effect or decreased maize sperm longevity and/or viability.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“whereas other buffers had no effect or decreased the longevity and/or viability”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
pH 6.7 was optimal among five tested pH values for maintaining maize sperm longevity and viability.
- Treatment
- pH 6.7e5
- Outcome
- maintenance of both longevity and viabilitye5
- Direction
- optimale5
- Comparator
- five pH values testede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Among the five pH values tested (5.0, 6.0, 6.7, 7.0, and 8.0), pH 6.7 appeared to be optimal for maintenance of both longevity and viability”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
Cysteine caused a rapid decrease in maize sperm cell viability.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“cysteine caused a rapid decrease in cell viability and increased lysis”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
Cysteine increased maize sperm cell lysis.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“cysteine caused a rapid decrease in cell viability and increased lysis”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
Dithiothreitol increased maize sperm cell numbers.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“dithiothreitol increased the cell numbers but lowered their viability”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
Dithiothreitol lowered maize sperm cell viability.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“dithiothreitol increased the cell numbers but lowered their viability”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
Addition of 0.1% bovine serum albumin increased maize sperm cell numbers and viability.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Addition of 0.1% bovine serum albumin increased cell numbers and viability”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
About 70% of maize sperm cells remained viable after 72 h of suspension.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“about 70% of the cells remained viable after 72 h of suspension”
t0“Isolated from Pollen of Zea mays L.”
Claim · model-written summary
Maize sperm longevity and viability improved in 0.44 m sucrose when the solution was conditioned with MES and bovine serum albumin.
- Treatment
- conditioned with 2-(N-morpholino)ethanesulfonic acid and bovine serum albumine2
- Outcome
- Cell longevity and viabilitye2
- Direction
- improvede2
- Qualifier
- 0.44 m sucrosee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Cell longevity and viability were also improved in 0.44 m sucrose when the solution was conditioned with 2-(N-morpholino)ethanesulfonic acid and bovine serum albumin”
t0“Isolated from Pollen of Zea mays L.”
The same questions in other papers
ph · pest · Zea mays (maize)Positive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.