Claim · model-written summary
Silencing chloroplast HSP90C downregulated genes involved in photosynthesis, plastid organization and cell cycle in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- silenced in an artificially inducible mannere0
- Outcome
- genes involved in photosynthesis, plastid organization, and cell cyclee4
- Direction
- downregulatione4
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner”
e4“the downregulation of genes involved in photosynthesis, plastid organization, and cell cycle”
Title
Shaikhul Islam; Sachin Ashok Bhor; Keisuke Tanaka; et al. International Journal of Molecular Sciences 2020. doi:10.3390/ijms21124202
Other claims from this paper (7)
- Intervention effect Silencing chloroplast HSP90C in tobacco upregulated 2746 genes.
- Intervention effect Silencing chloroplast HSP90C in tobacco downregulated 3490 genes.
- Intervention effect Silencing chloroplast HSP90C upregulated ROS-responsive genes in tobacco.
- Intervention effect Silencing chloroplast HSP90C activated innate immunity and cell death pathways in tobacco.
- Observation Cell death was confirmed in the silenced tobacco plants by trypan blue staining and electrolyte leakage.
- Observation H2O2 production was confirmed in the silenced tobacco plants by diaminobenzidine staining.
- Mechanism Reduced HSP90C chaperone levels lead tobacco to develop chlorosis mainly through down-regulation of chloroplast- and photosynthesis-related genes, and additionally through light-dependent ROS production and immune activation.