Abstract
RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner resulting in the development of chlorosis, revealed the up- and downregulation of 2746 and 3490 genes, respectively. Gene ontology analysis of these differentially expressed genes indicated the upregulation of ROS-responsive genes; the activation of the innate immunity and cell death pathways; and the downregulation of genes involved in photosynthesis, plastid organization, and cell cycle. Cell death was confirmed by trypan blue staining and electrolyte leakage assay, and the H2O2 production was confirmed by diaminobenzidine staining. The results collectively suggest that the reduced levels of HSP90C chaperone lead the plant to develop chlorosis primarily through the global downregulation of chloroplast- and photosynthesis-related genes and additionally through the light-dependent production of ROS, followed by the activation of immune responses, including cell death.
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Claims 8
Claim · model-written summary
Silencing chloroplast HSP90C in tobacco upregulated 2746 genes.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- silenced in an artificially inducible mannere0
- Outcome
- differentially expressed genese2
- Direction
- increaseinferrede1
- Value
- 2746e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner”
e1“revealed the up- and downregulation of 2746 and 3490 genes, respectively”
e2“Gene ontology analysis of these differentially expressed genes indicated the upregulation of ROS-responsive genes”
Claim · model-written summary
Silencing chloroplast HSP90C in tobacco downregulated 3490 genes.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- silenced in an artificially inducible mannere0
- Outcome
- differentially expressed genese2
- Direction
- decreaseinferrede1
- Value
- 3490e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner”
e1“revealed the up- and downregulation of 2746 and 3490 genes, respectively”
e2“Gene ontology analysis of these differentially expressed genes indicated the upregulation of ROS-responsive genes”
Claim · model-written summary
Silencing chloroplast HSP90C upregulated ROS-responsive genes in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- silenced in an artificially inducible mannere0
- Outcome
- ROS-responsive genese2
- Direction
- upregulatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner”
e2“Gene ontology analysis of these differentially expressed genes indicated the upregulation of ROS-responsive genes”
Claim · model-written summary
Silencing chloroplast HSP90C activated innate immunity and cell death pathways in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- silenced in an artificially inducible mannere0
- Outcome
- innate immunity and cell death pathwayse3
- Direction
- activatione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner”
e3“the activation of the innate immunity and cell death pathways”
Claim · model-written summary
Silencing chloroplast HSP90C downregulated genes involved in photosynthesis, plastid organization and cell cycle in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- silenced in an artificially inducible mannere0
- Outcome
- genes involved in photosynthesis, plastid organization, and cell cyclee4
- Direction
- downregulatione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner”
e4“the downregulation of genes involved in photosynthesis, plastid organization, and cell cycle”
Claim · model-written summary
Cell death was confirmed in the silenced tobacco plants by trypan blue staining and electrolyte leakage.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- Cell deathe5
- Qualifier
- trypan blue staining and electrolyte leakage assaye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner”
e5“Cell death was confirmed by trypan blue staining and electrolyte leakage assay”
Claim · model-written summary
H2O2 production was confirmed in the silenced tobacco plants by diaminobenzidine staining.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- H2O2 productione6
- Qualifier
- diaminobenzidine staininge6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner”
e6“the H2O2 production was confirmed by diaminobenzidine staining”
Claim · model-written summary
Reduced HSP90C chaperone levels lead tobacco to develop chlorosis mainly through down-regulation of chloroplast- and photosynthesis-related genes, and additionally through light-dependent ROS production and immune activation.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- HSP90Ce7
- Outcome
- chlorosise7
- Qualifier
- the global downregulation of chloroplast- and photosynthesis-related genese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner”
e7“The results collectively suggest that the reduced levels of HSP90C chaperone lead the plant to develop chlorosis primarily through the global downregulation of chloroplast- and photosynthesis-related genes”