Claim · model-written summary
Medium acidification during autoclaving contributed only a minor effect.
- Taxon
- CruciferaeBrassicaceaee1
- Outcome
- acidification of the mediume0
- Qualifier
- only minor importancee0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Verbatim
e0“The acidification of the medium during autoclaving was of only minor importance”
e1“fructose media sterilized by filtration are clearly beneficial for the growth of intact plants of Cruciferae”
Title
‘Fructose Effect’ in Higher Plants*
RÉDEI G. P. Annals of Botany 1974. doi:10.1093/oxfordjournals.aob.a084812
Other claims from this paper (12)
- Intervention effect Filter-sterilized fructose medium benefited growth of intact Cruciferae.
- Observation Fructose decomposed faster during autoclaving than glucose or sucrose.
- Mechanism Plants suffered on autoclaved fructose media because toxic degradation products accumulated.
- Observation Early fructose breakdown products absorbing near 230 nm were apparently harmless.
- Intervention effect Prolonged heating shifted the absorption maximum to around 285 nm.
- Observation 5-hydroxymethyl-2-furaldehyde was toxic in the 10-4 M range.
- Observation Hydroxyacetyl furan was toxic in the 10-4 M range.
- Method / resource Thin layer chromatography separated at least ten decomposition-product hydrazones.
- Intervention effect Cyclic adenosine monophosphate did not relieve toxicity of autoclaved fructose.
- Association The adverse fructose-medium effect in crucifers was unrelated to sugar chelation.
- Mechanism 5-hydroxymethyl-2- furaldehyde was identified as the primary cause of autoclaved fructose toxicity.
- Mechanism Other decomposition products added to the toxicity of autoclaved fructose.