Claim · model-written summary
Reduced glutathione did not elicit endogenous CHS activity in untreated protoplasts.
- Taxon
- alfalfa (Medicago sativa L.)
Medicago sativae0 - Treatment
- reduced glutathione (GSH)e0
- Outcome
- chalcone synthase (CHS) activitye0
- Direction
- unresponsivee0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protoplasts derived from cell suspensions of alfalfa (Medicago sativa L.) responded to treatment with fungal elicitor (FE) by an increase in endogenous chalcone synthase (CHS) activity but were unresponsive to reduced glutathione (GSH).”
Title
Stress Responses in Alfalfa (Medicago sativa L.)
Arvind D. Choudhary; Christopher J. Lamb; Richard A. Dixon PLANT PHYSIOLOGY 1990. doi:10.1104/pp.94.4.1802
Other claims from this paper (10)
- Intervention effect Fungal elicitor increased endogenous CHS activity in alfalfa protoplasts.
- Intervention effect Polyethylene glycol preexposure and electroporation induced strong GSH responsiveness.
- Intervention effect Polyethylene glycol preexposure and electroporation caused little change in FE responsiveness.
- Observation After more than 12 subcultures, protoplasts lost GSH responsiveness measured by reporter expression.
- Observation After more than 12 subcultures, protoplasts retained FE responsiveness.
- Observation CAT expression depended on elicitor, subculture age, and coelectroporated CHS promoter sequence.
- Observation The CHS promoter region -326 to -141 acted as a trans-activator in unelicited protoplasts from new suspensions.
- Observation The same CHS region acted as a trans-silencer under fungal elicitor.
- Intervention effect GSH treatment much reduced that silencer activity.
- Mechanism Authors infer distinct signal transduction pathways for FE and GSH elicitation of CHS transcription.