Claim · model-written summary
Chitin-containing fungal cell walls induced C3 chitinase production in vitro.
- Taxon
- tall fescue
Lolium arundinaceumt0 - Treatment
- chitin-containing fungal cell wallse0
- Interactor
- C3e0biocontrol_agent
- Outcome
- Chitinase production by C3e0
- Direction
- inducede0
- Setting
- in vitroe0
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleComparatorValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chitinase production by C3 was induced in vitro by chitin or chitin-containing fungal cell walls”
t0“tall fescue”
Title
Zhongge Zhang Lincoln (University of Nebraska) 1999
Other claims from this paper (19)
- Intervention effect C3 inhibited B. sorokiniana conidial germination on tall fescue leaves.
- Intervention effect C3 inhibited B. sorokiniana germ tube growth on tall fescue leaves.
- Intervention effect C3 reduced B. sorokiniana leaf spot incidence on tall fescue in growth chambers and fields.
- Intervention effect C3 reduced B. sorokiniana leaf spot severity on tall fescue in growth chambers and fields.
- Mechanism Chitinolysis was identified as one mechanism of C3 action.
- Intervention effect Chitinase-deficient or low-chitinase C3 mutants colonized grass less than wild type.
- Intervention effect Chitinase-deficient or low-chitinase C3 mutants provided less disease protection than wild type.
- Intervention effect C3 colonization raised chitinase activity on grass leaves.
- Intervention effect C3 colonization raised chitinase activity on bean blossoms.
- Intervention effect Adding chitin with C3 further increased chitinase activity on plant parts.
- Intervention effect A chitinase-minus C3 mutant gave no added chitinase activity on plant parts, with or without chitin.
- Association Extracts from C3-colonized plant parts had antifungal activity associated with chitinase activity.
- Intervention effect Chitin induced C3 chitinase production in vitro.
- Intervention effect C3 chitinolytic fractions were more antifungal than non-chitinolytic fractions.
- Observation C3 chitinolytic fractions had strong exochitinase and slight endochitinase activities.
- Observation The major chitinolytic C3 fraction revealed five proteins of 25, 32, 48, 65 and 75 KDa.
- Observation Only the 32-Kda protein N-terminus showed homology to known bacterial chitinases.
- Mechanism Five chitinase-active C3 bands reduced to 32-Kda and 48-Kda proteins after denaturing re-electrophoresis, suggesting cleavage products.
- Intervention effect Combining C3 cells with chitin, chitin-broth fluid, or chitinase induction increased disease control efficacy.