Claim · model-written summary
Modified enzyme does not migrate in electrophoresis.
Zea maysNot reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e0“Three isozyme variants of catechol oxidase have been shown to be determined by alleles of a gene, Cx, which has been located on chromosome 10 less than 0.1 recombination units from the endosperm marker du(1)”
e3“Modified enzyme has altered kinetics, does not migrate in electrophoresis and most probably results from a "tanning" of the enzyme by reaction products”
Title
THE GENETIC CONTROL AND BIOCHEMICAL MODIFICATION OF CATECHOL OXIDASE IN MAIZE
Tony Pryor; Drew Schwartz Genetics 1973. doi:10.1093/genetics/75.1.75
Other claims from this paper (12)
- Observation Three catechol oxidase isozyme variants are determined by alleles of gene Cx.
- Observation Gene Cx is located on chromosome 10 less than 0.1 recombination units from the endosperm marker du(1).
- Mechanism The extractable form of the enzyme is modified by an endogenous modifier that appears to function as an enzyme substrate.
- Observation Modified enzyme has altered kinetics.
- Mechanism Modified enzyme most probably results from tanning of the enzyme by reaction products.
- Intervention effect Maleic hydrazide causes a ten-fold reduction in the modifier content of seedlings.
- Intervention effect Maleic hydrazide allows the enzyme to be extracted in an unmodified form.
- Observation The unmodified enzyme will migrate in electrophoresis.
- Observation Enzyme and modifier are functionally isolated in intact cells.
- Observation The enzyme and modifier system fits the requirements of hypersensitive disease resistance in plants.
- Observation The system may provide a test system to investigate the biochemical basis of disease resistance.
- Observation The content of modifier varies in different lines and is genetically determined by genes independent of Cx.