Abstract
Three isozyme variants of catechol oxidase have been shown to be determined by alleles of a gene, Cx, which has been located on chromosome 10 less than 0.1 recombination units from the endosperm marker du(1).-The extractable form of the enzyme is modified by an endogeneous "modifier" which appears to function as an enzyme substrate. Enzyme and modifier are functionally isolated in intact cells. Modified enzyme has altered kinetics, does not migrate in electrophoresis and most probably results from a "tanning" of the enzyme by reaction products. The content of modifier varies in different lines and is genetically determined by gene(s) independent of Cx. Treatment with maleic hydrazide causes a ten-fold reduction in the modifier content of seedlings, allowing the enzyme to be extracted in an unmodified form which will migrate in electrophoresis.-This system of enzyme and modifier fits the requirements of hypersensitive disease resistance in plants and may provide a test system to investigate the biochemical basis of disease resistance.
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Claims 13
Claim · model-written summary
Three catechol oxidase isozyme variants are determined by alleles of gene Cx.
Zea maysNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e0“Three isozyme variants of catechol oxidase have been shown to be determined by alleles of a gene, Cx, which has been located on chromosome 10 less than 0.1 recombination units from the endosperm marker du(1)”
Claim · model-written summary
Gene Cx is located on chromosome 10 less than 0.1 recombination units from the endosperm marker du(1).
- Gene
- Cxe0
- Outcome
- located on chromosome 10e0
- Value
- less than 0.1 recombination unitse0
- Comparator
- endosperm marker du(1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e0“Three isozyme variants of catechol oxidase have been shown to be determined by alleles of a gene, Cx, which has been located on chromosome 10 less than 0.1 recombination units from the endosperm marker du(1)”
Claim · model-written summary
The extractable form of the enzyme is modified by an endogenous modifier that appears to function as an enzyme substrate.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e1“The extractable form of the enzyme is modified by an endogeneous "modifier" which appears to function as an enzyme substrate”
Claim · model-written summary
Modified enzyme has altered kinetics.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e0“Three isozyme variants of catechol oxidase have been shown to be determined by alleles of a gene, Cx, which has been located on chromosome 10 less than 0.1 recombination units from the endosperm marker du(1)”
e3“Modified enzyme has altered kinetics, does not migrate in electrophoresis and most probably results from a "tanning" of the enzyme by reaction products”
Claim · model-written summary
Modified enzyme does not migrate in electrophoresis.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e0“Three isozyme variants of catechol oxidase have been shown to be determined by alleles of a gene, Cx, which has been located on chromosome 10 less than 0.1 recombination units from the endosperm marker du(1)”
e3“Modified enzyme has altered kinetics, does not migrate in electrophoresis and most probably results from a "tanning" of the enzyme by reaction products”
Claim · model-written summary
Modified enzyme most probably results from tanning of the enzyme by reaction products.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e3“Modified enzyme has altered kinetics, does not migrate in electrophoresis and most probably results from a "tanning" of the enzyme by reaction products”
Claim · model-written summary
Maleic hydrazide causes a ten-fold reduction in the modifier content of seedlings.
- Organ / stage
- seedlingse5
- Treatment
- maleic hydrazidee5
- Outcome
- modifier content of seedlingse5
- Direction
- reductione5
- Value
- ten-folde5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e5“Treatment with maleic hydrazide causes a ten-fold reduction in the modifier content of seedlings, allowing the enzyme to be extracted in an unmodified form which will migrate in electrophoresis”
Claim · model-written summary
Maleic hydrazide allows the enzyme to be extracted in an unmodified form.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e5“Treatment with maleic hydrazide causes a ten-fold reduction in the modifier content of seedlings, allowing the enzyme to be extracted in an unmodified form which will migrate in electrophoresis”
Claim · model-written summary
The unmodified enzyme will migrate in electrophoresis.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e5“Treatment with maleic hydrazide causes a ten-fold reduction in the modifier content of seedlings, allowing the enzyme to be extracted in an unmodified form which will migrate in electrophoresis”
Claim · model-written summary
Enzyme and modifier are functionally isolated in intact cells.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e2“Enzyme and modifier are functionally isolated in intact cells”
Claim · model-written summary
The enzyme and modifier system fits the requirements of hypersensitive disease resistance in plants.
- Outcome
- fits the requirements of hypersensitive disease resistance in plantse6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e6“This system of enzyme and modifier fits the requirements of hypersensitive disease resistance in plants and may provide a test system to investigate the biochemical basis of disease resistance”
Claim · model-written summary
The system may provide a test system to investigate the biochemical basis of disease resistance.
- Outcome
- test system to investigate the biochemical basis of disease resistancee6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e6“This system of enzyme and modifier fits the requirements of hypersensitive disease resistance in plants and may provide a test system to investigate the biochemical basis of disease resistance”
Claim · model-written summary
The content of modifier varies in different lines and is genetically determined by genes independent of Cx.
- Material
- different linese4
- Outcome
- content of modifiere4
- Direction
- variese4
- Qualifier
- genetically determined by gene(s) independent of Cxe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CATECHOL OXIDASE IN MAIZE”
e4“The content of modifier varies in different lines and is genetically determined by gene(s) independent of Cx”