By organism › Green plants › Ranunculaceae › Helleborus orientalis

Observationobservedoriginal resultmeasured

Claim · model-written summary

Peronospora and Plasmopara occurred on Lenten rose in North Carolina and Virginia.

Taxon: Lenten roseHelleborus orientalise0
Outcome: Lenten rosee0
Setting: North Carolina, Virginiae0

Verbatim

e0
“Lenten rose (North Carolina, Virginia)”
✓ located verbatim in the source · abstract, characters 988–1026

Title

Where Flowers Bloom, so Do Downy Mildews: New Species and New Records of Hyaloperonospora , Peronospora , and Plasmopara Species on Ornamental and Wild Plants in the United States

Catalina Salgado-Salazar; Lina M. Rodríguez Salamanca; Megan K. Romberg; et al. Plant Health Progress 2025. doi:10.1094/php-10-24-0100-rs

Other claims from this paper (10)

Observation Peronospora and Plasmopara occurred on frost grape in Ohio.
Observation Peronospora and Plasmopara occurred on mock strawberry in Maryland.
Observation Peronospora and Plasmopara occurred on redbud in North Carolina.
Observation Peronospora and Plasmopara occurred on snapdragon in Virginia.
Observation Peronospora and Plasmopara occurred on viburnum in North Carolina.
Observation Peronospora and Plasmopara occurred on Virginia creeper in Maryland and Ohio.
Observation Snapdragon Peronospora isolates were separate from isolates on Misopates and described as Pe. ceperoae.
Observation Peronospora matthiolae on stock was transferred to Hyaloperonospora.
Observation The authors infer wider geographic distributions for the reported downy mildews.
Recommendation The authors recommend continuous downy mildew surveillance.

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

About 10% of hellebores in the garden had symptoms.

Taxon: Helleborus orientalisHelleborus orientalist0
Outcome: exhibited symptomse0
Value: 10%e0
Setting: gardene0

Verbatim

e0
“approximately 10% of the hellebore population in the garden exhibited symptoms”
✓ located verbatim in the source · abstract, characters 532–610

t0
“Helleborus orientalis”
✓ located verbatim in the source · title, characters 88–109

Title

First Report of Helleborus Net Necrosis Virus ( Carlavirus necroretis ) Associated with Helleborus orientalis in Australia

Ossie Wildman; Mukesh Srivastava; Solomon Maina Plant Disease 2025. doi:10.1094/pdis-06-25-1326-pdn

Other claims from this paper (6)

Observationobservedoriginal resultmeasured

Claim · model-written summary

Electron microscopy found 650-700 nm filamentous virus-like particles.

Taxon: Helleborus orientalisHelleborus orientalist0
Outcome: filamentous virus-like particlese0
Value: 650-700 nme0

Verbatim

e0
“electron microscopy analysis revealed filamentous virus-like particles measuring 650-700 nm length”
✓ located verbatim in the source · abstract, characters 1242–1340

t0
“Helleborus orientalis”
✓ located verbatim in the source · title, characters 88–109

Title

First Report of Helleborus Net Necrosis Virus ( Carlavirus necroretis ) Associated with Helleborus orientalis in Australia

Ossie Wildman; Mukesh Srivastava; Solomon Maina Plant Disease 2025. doi:10.1094/pdis-06-25-1326-pdn

Other claims from this paper (6)

Observation About 10% of hellebores in the garden had symptoms.
Observation Sequencing produced a viral contig of 8,534 nucleotides with 99.3% identity to a HeNNV genome.
Observation The viral contig comprised 2.6% of reads and had 1336x average genome coverage.
Method / resource RT-PCR and Sanger sequencing yielded a 604 bp amplicon identical to the sequencing contig.
Observation The authors report the first detection of HeNNV infecting hellebores in Australia.
Recommendation The authors urge stringent screening of imported plant propagules after detecting HeNNV.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Sequencing produced a viral contig of 8,534 nucleotides with 99.3% identity to a HeNNV genome.

Taxon: Helleborus orientalisHelleborus orientalist0
Interactor: HeNNVe0pathogen
Outcome: sequence identity with a HeNNV genomee0
Value: 99.3%e0
Qualifier: 8,534 nucleotides (nt)e0

Verbatim

e0
“a single contig of 8,534 nucleotides (nt) sharing 99.3% sequence identity with a HeNNV genome”
✓ located verbatim in the source · abstract, characters 2111–2204

t0
“Helleborus orientalis”
✓ located verbatim in the source · title, characters 88–109

Title

First Report of Helleborus Net Necrosis Virus ( Carlavirus necroretis ) Associated with Helleborus orientalis in Australia

Ossie Wildman; Mukesh Srivastava; Solomon Maina Plant Disease 2025. doi:10.1094/pdis-06-25-1326-pdn

Other claims from this paper (6)

Observation About 10% of hellebores in the garden had symptoms.
Observation Electron microscopy found 650-700 nm filamentous virus-like particles.
Observation The viral contig comprised 2.6% of reads and had 1336x average genome coverage.
Method / resource RT-PCR and Sanger sequencing yielded a 604 bp amplicon identical to the sequencing contig.
Observation The authors report the first detection of HeNNV infecting hellebores in Australia.
Recommendation The authors urge stringent screening of imported plant propagules after detecting HeNNV.

Observationobservedoriginal resultmeasured

Claim · model-written summary

The viral contig comprised 2.6% of reads and had 1336x average genome coverage.

Taxon: Helleborus orientalisHelleborus orientalist0
Outcome: average genome coveragee0
Value: 1336xe0
Qualifier: 2.6% of the total readse0

Verbatim

e0
“The contig of interest was represented by 2.6% of the total reads (79,136 reads out of 3,006,106 total reads), giving an average genome coverage of 1336x”
✓ located verbatim in the source · abstract, characters 2251–2404

t0
“Helleborus orientalis”
✓ located verbatim in the source · title, characters 88–109

Title

First Report of Helleborus Net Necrosis Virus ( Carlavirus necroretis ) Associated with Helleborus orientalis in Australia

Ossie Wildman; Mukesh Srivastava; Solomon Maina Plant Disease 2025. doi:10.1094/pdis-06-25-1326-pdn

Other claims from this paper (6)

Observation About 10% of hellebores in the garden had symptoms.
Observation Electron microscopy found 650-700 nm filamentous virus-like particles.
Observation Sequencing produced a viral contig of 8,534 nucleotides with 99.3% identity to a HeNNV genome.
Method / resource RT-PCR and Sanger sequencing yielded a 604 bp amplicon identical to the sequencing contig.
Observation The authors report the first detection of HeNNV infecting hellebores in Australia.
Recommendation The authors urge stringent screening of imported plant propagules after detecting HeNNV.

Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors urge stringent screening of imported plant propagules after detecting HeNNV.

Taxon: Helleborus orientalisHelleborus orientalist0
Interactor: HeNNVe0pathogen
Outcome: stringent biosecurity screening of imported plant propagulese0
Setting: Australiae0

Verbatim

e0
“This HeNNV detection reinforces the importance of stringent biosecurity screening of imported plant propagules entering Australia”
✓ located verbatim in the source · abstract, characters 3143–3272

t0
“Helleborus orientalis”
✓ located verbatim in the source · title, characters 88–109

Title

First Report of Helleborus Net Necrosis Virus ( Carlavirus necroretis ) Associated with Helleborus orientalis in Australia

Ossie Wildman; Mukesh Srivastava; Solomon Maina Plant Disease 2025. doi:10.1094/pdis-06-25-1326-pdn

Other claims from this paper (6)

Observation About 10% of hellebores in the garden had symptoms.
Observation Electron microscopy found 650-700 nm filamentous virus-like particles.
Observation Sequencing produced a viral contig of 8,534 nucleotides with 99.3% identity to a HeNNV genome.
Observation The viral contig comprised 2.6% of reads and had 1336x average genome coverage.
Method / resource RT-PCR and Sanger sequencing yielded a 604 bp amplicon identical to the sequencing contig.
Observation The authors report the first detection of HeNNV infecting hellebores in Australia.

Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Calcium carbonate dose significantly affected plant height regardless of plant age.

Taxon: lenten roseHelleborus orientalist0
Treatment: CaCO 3 dosee0
Outcome: plant heighte0

Not reportedComparatorDirection

Verbatim

e0
“The CaCO 3 dose applied to deacidify high moor peat had a significant effect on plant height irrespective of their age”
✓ located verbatim in the source · abstract, characters 440–558

t0
“lenten rose (Helleborus orientalis Lam.)”
✓ located verbatim in the source · title, characters 24–64

Title

Growth and flowering of lenten rose (Helleborus orientalis Lam.) depending on the dose of calcium carbonate and top dressing with Peters Professional Special

M. Henschke; P. Czuchaj Acta Scientiarum Polonorum Hortorum Cultus 2008

Other claims from this paper (8)

Intervention effect At 2.5 g #dm -3 calcium carbonate, plants were tallest in both cultivation years.
Intervention effect At 10 g #dm -3 calcium carbonate, plants were shortest in both cultivation years.
Intervention effect Leaf number did not depend on calcium carbonate amount.
Intervention effect Flowering did not depend on calcium carbonate amount.
Intervention effect Higher fertilizer concentration produced taller plants.
Intervention effect Higher fertilizer concentration produced more leaves.
Intervention effect Higher fertilizer concentration produced earlier flowering.
Intervention effect Higher fertilizer concentration produced more abundant flowering.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 2.5 g #dm -3 calcium carbonate, plants were tallest in both cultivation years.

Taxon: lenten roseHelleborus orientalist0
Treatment: calcium carbonate was added at 2.5 g #dm -3 CaCO 3e0
Outcome: tallest plantse0
Direction: talleste0
Qualifier: first and second year of pot cultivatione0
Comparator: 10 g #dm -3 CaCO 3e0

Verbatim

e0
“In the first and second year of pot cultivation the tallest plants were produced on a substrate to which calcium carbonate was added at 2.5 g #dm -3 CaCO 3, while the lowest at 10 g #dm -3 CaCO 3”
✓ located verbatim in the source · abstract, characters 560–755

t0
“lenten rose (Helleborus orientalis Lam.)”
✓ located verbatim in the source · title, characters 24–64

Title

Growth and flowering of lenten rose (Helleborus orientalis Lam.) depending on the dose of calcium carbonate and top dressing with Peters Professional Special

M. Henschke; P. Czuchaj Acta Scientiarum Polonorum Hortorum Cultus 2008

Other claims from this paper (8)

Intervention effect Calcium carbonate dose significantly affected plant height regardless of plant age.
Intervention effect At 10 g #dm -3 calcium carbonate, plants were shortest in both cultivation years.
Intervention effect Leaf number did not depend on calcium carbonate amount.
Intervention effect Flowering did not depend on calcium carbonate amount.
Intervention effect Higher fertilizer concentration produced taller plants.
Intervention effect Higher fertilizer concentration produced more leaves.
Intervention effect Higher fertilizer concentration produced earlier flowering.
Intervention effect Higher fertilizer concentration produced more abundant flowering.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At 10 g #dm -3 calcium carbonate, plants were shortest in both cultivation years.

Taxon: lenten roseHelleborus orientalist0
Treatment: 10 g #dm -3 CaCO 3e0
Outcome: loweste0
Direction: loweste0
Qualifier: first and second year of pot cultivatione0
Comparator: 2.5 g #dm -3 CaCO 3e0

Verbatim

e0
“In the first and second year of pot cultivation the tallest plants were produced on a substrate to which calcium carbonate was added at 2.5 g #dm -3 CaCO 3, while the lowest at 10 g #dm -3 CaCO 3”
✓ located verbatim in the source · abstract, characters 560–755

t0
“lenten rose (Helleborus orientalis Lam.)”
✓ located verbatim in the source · title, characters 24–64

Title

Growth and flowering of lenten rose (Helleborus orientalis Lam.) depending on the dose of calcium carbonate and top dressing with Peters Professional Special

M. Henschke; P. Czuchaj Acta Scientiarum Polonorum Hortorum Cultus 2008

Other claims from this paper (8)

Intervention effect Calcium carbonate dose significantly affected plant height regardless of plant age.
Intervention effect At 2.5 g #dm -3 calcium carbonate, plants were tallest in both cultivation years.
Intervention effect Leaf number did not depend on calcium carbonate amount.
Intervention effect Flowering did not depend on calcium carbonate amount.
Intervention effect Higher fertilizer concentration produced taller plants.
Intervention effect Higher fertilizer concentration produced more leaves.
Intervention effect Higher fertilizer concentration produced earlier flowering.
Intervention effect Higher fertilizer concentration produced more abundant flowering.

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Leaf number did not depend on calcium carbonate amount.

Taxon: lenten roseHelleborus orientalist0
Treatment: amount of calcium carbonatee0
Outcome: number of leavese0
Direction: did not depende0

Not reportedComparator

Verbatim

e0
“The number of leaves and flow- ering of lenten rose did not depend on the amount of calcium carbonate introduced to the substrate”
✓ located verbatim in the source · abstract, characters 757–886

t0
“lenten rose (Helleborus orientalis Lam.)”
✓ located verbatim in the source · title, characters 24–64

Title

Growth and flowering of lenten rose (Helleborus orientalis Lam.) depending on the dose of calcium carbonate and top dressing with Peters Professional Special

M. Henschke; P. Czuchaj Acta Scientiarum Polonorum Hortorum Cultus 2008

Other claims from this paper (8)

Intervention effect Calcium carbonate dose significantly affected plant height regardless of plant age.
Intervention effect At 2.5 g #dm -3 calcium carbonate, plants were tallest in both cultivation years.
Intervention effect At 10 g #dm -3 calcium carbonate, plants were shortest in both cultivation years.
Intervention effect Flowering did not depend on calcium carbonate amount.
Intervention effect Higher fertilizer concentration produced taller plants.
Intervention effect Higher fertilizer concentration produced more leaves.
Intervention effect Higher fertilizer concentration produced earlier flowering.
Intervention effect Higher fertilizer concentration produced more abundant flowering.

Helleborus orientalis 19 claims

19 claims