Syzygium aromaticum 1,757 claims

species · family Myrtaceae · green plant · NCBI Taxonomy ↗

Common name: clove — Also written in claims: clove · cloves · clove trees · clove tree · Eugenia caryophyllata · clove seedlings — NCBI synonyms: Caryophyllus aromaticus · Eugenia aromatica · Eugenia caryophyllata

63 spellings

Questions studied on this organism10 questions17 papers

Syzygium aromaticumBayu Nugroho · CC BY-SA 4.0 · Wikimedia Commons ↗

1,757 claims

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Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The clove genome contains 189,127 SSRs identified in silico.

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: SSRse1
Value: 189,127e1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“An in silico survey of the 367.77 Mb genome assembly identified 189,127 SSRs at an overall frequency of 514.25 loci/Mb”
✓ located verbatim in the source · abstract, characters 319–437

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome SSR overall frequency is 514.25 loci/Mb.
Observation Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).
Observation Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).
Observation The clove SSRs show a strong A/T-rich bias.
Observation The clove SSRs show a notable scarcity of CG/GC motifs.
Observation SSRs are uniformly distributed at the whole-chromosome level in clove.
Observation SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.
Observation The clove genome has 11,107 hypervariable Class I SSR loci.
Observation Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The clove genome SSR overall frequency is 514.25 loci/Mb.

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: overall frequencye1
Value: 514.25 loci/Mbe1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“An in silico survey of the 367.77 Mb genome assembly identified 189,127 SSRs at an overall frequency of 514.25 loci/Mb”
✓ located verbatim in the source · abstract, characters 319–437

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome contains 189,127 SSRs identified in silico.
Observation Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).
Observation Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).
Observation The clove SSRs show a strong A/T-rich bias.
Observation The clove SSRs show a notable scarcity of CG/GC motifs.
Observation SSRs are uniformly distributed at the whole-chromosome level in clove.
Observation SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.
Observation The clove genome has 11,107 hypervariable Class I SSR loci.
Observation Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: Mononucleotidese1
Value: 50.30%e1
Qualifier: most abundant repeat typese1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“Mononucleotides (50.30%) and dinucleotides (40.68%) were the most abundant repeat types, exhibiting a strong A/T-rich bias and a notable scarcity of CG/GC motifs”
✓ located verbatim in the source · abstract, characters 439–600

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome contains 189,127 SSRs identified in silico.
Method / resource The clove genome SSR overall frequency is 514.25 loci/Mb.
Observation Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).
Observation The clove SSRs show a strong A/T-rich bias.
Observation The clove SSRs show a notable scarcity of CG/GC motifs.
Observation SSRs are uniformly distributed at the whole-chromosome level in clove.
Observation SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.
Observation The clove genome has 11,107 hypervariable Class I SSR loci.
Observation Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: dinucleotidese1
Value: 40.68%e1
Qualifier: most abundant repeat typese1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“Mononucleotides (50.30%) and dinucleotides (40.68%) were the most abundant repeat types, exhibiting a strong A/T-rich bias and a notable scarcity of CG/GC motifs”
✓ located verbatim in the source · abstract, characters 439–600

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome contains 189,127 SSRs identified in silico.
Method / resource The clove genome SSR overall frequency is 514.25 loci/Mb.
Observation Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).
Observation The clove SSRs show a strong A/T-rich bias.
Observation The clove SSRs show a notable scarcity of CG/GC motifs.
Observation SSRs are uniformly distributed at the whole-chromosome level in clove.
Observation SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.
Observation The clove genome has 11,107 hypervariable Class I SSR loci.
Observation Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.

Observationobservedoriginal resultmeasured

Claim · model-written summary

The clove SSRs show a strong A/T-rich bias.

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: A/T-rich biase1
Qualifier: stronge1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“Mononucleotides (50.30%) and dinucleotides (40.68%) were the most abundant repeat types, exhibiting a strong A/T-rich bias and a notable scarcity of CG/GC motifs”
✓ located verbatim in the source · abstract, characters 439–600

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome contains 189,127 SSRs identified in silico.
Method / resource The clove genome SSR overall frequency is 514.25 loci/Mb.
Observation Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).
Observation Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).
Observation The clove SSRs show a notable scarcity of CG/GC motifs.
Observation SSRs are uniformly distributed at the whole-chromosome level in clove.
Observation SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.
Observation The clove genome has 11,107 hypervariable Class I SSR loci.
Observation Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.

Observationobservedoriginal resultmeasured

Claim · model-written summary

The clove SSRs show a notable scarcity of CG/GC motifs.

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: scarcity of CG/GC motifse1
Qualifier: notablee1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“Mononucleotides (50.30%) and dinucleotides (40.68%) were the most abundant repeat types, exhibiting a strong A/T-rich bias and a notable scarcity of CG/GC motifs”
✓ located verbatim in the source · abstract, characters 439–600

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome contains 189,127 SSRs identified in silico.
Method / resource The clove genome SSR overall frequency is 514.25 loci/Mb.
Observation Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).
Observation Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).
Observation The clove SSRs show a strong A/T-rich bias.
Observation SSRs are uniformly distributed at the whole-chromosome level in clove.
Observation SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.
Observation The clove genome has 11,107 hypervariable Class I SSR loci.
Observation Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.

Observationobservedoriginal resultmeasured

Claim · model-written summary

SSRs are uniformly distributed at the whole-chromosome level in clove.

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: uniformly distributed at the whole-chromosome levele1
Qualifier: R² = 0.99 relative to chromosome lengthe1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“Although SSRs were uniformly distributed at the whole-chromosome level (R² = 0.99 relative to chromosome length)”
✓ located verbatim in the source · abstract, characters 602–714

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome contains 189,127 SSRs identified in silico.
Method / resource The clove genome SSR overall frequency is 514.25 loci/Mb.
Observation Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).
Observation Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).
Observation The clove SSRs show a strong A/T-rich bias.
Observation The clove SSRs show a notable scarcity of CG/GC motifs.
Observation SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.
Observation The clove genome has 11,107 hypervariable Class I SSR loci.
Observation Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.

Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: intra-chromosomal heterogeneitye1
Qualifier: hotspots (> 600 loci/Mb) and coldspots (< 400 loci/Mb)e1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“high-resolution analysis revealed significant intra-chromosomal heterogeneity, including distinct hotspots (> 600 loci/Mb) and coldspots (< 400 loci/Mb)”
✓ located verbatim in the source · abstract, characters 716–868

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome contains 189,127 SSRs identified in silico.
Method / resource The clove genome SSR overall frequency is 514.25 loci/Mb.
Observation Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).
Observation Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).
Observation The clove SSRs show a strong A/T-rich bias.
Observation The clove SSRs show a notable scarcity of CG/GC motifs.
Observation SSRs are uniformly distributed at the whole-chromosome level in clove.
Observation The clove genome has 11,107 hypervariable Class I SSR loci.
Observation Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.

Observationobservedoriginal resultmeasured

Claim · model-written summary

The clove genome has 11,107 hypervariable Class I SSR loci.

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: hypervariable Class I locie1
Value: 11,107e1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“A total of 11,107 hypervariable Class I loci were identified, predominantly comprising dinucleotide repeats (92.3%)”
✓ located verbatim in the source · abstract, characters 870–985

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome contains 189,127 SSRs identified in silico.
Method / resource The clove genome SSR overall frequency is 514.25 loci/Mb.
Observation Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).
Observation Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).
Observation The clove SSRs show a strong A/T-rich bias.
Observation The clove SSRs show a notable scarcity of CG/GC motifs.
Observation SSRs are uniformly distributed at the whole-chromosome level in clove.
Observation SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.
Observation Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Dinucleotide repeats make up 92.3% of the hypervariable Class I loci in clove.

Taxon: clove (Syzygium aromaticum)Syzygium aromaticume0
Outcome: dinucleotide repeatse1
Value: 92.3%e1
Qualifier: predominantly comprisinge1

Verbatim

e0
“Genetic improvement of clove (Syzygium aromaticum)”
✓ located verbatim in the source · abstract, characters 0–50

e1
“A total of 11,107 hypervariable Class I loci were identified, predominantly comprising dinucleotide repeats (92.3%)”
✓ located verbatim in the source · abstract, characters 870–985

Title

Genome-wide discovery, characterization, and validation of novel SSR markers in clove (Syzygium aromaticum) and their application in genetic analysis.

Muhammed Azharudheen T P; Muhammed Nissar V A; Anees K; et al. PubMed 2026. doi:10.1186/s12870-026-09078-5

Other claims from this paper (19)

Method / resource The clove genome contains 189,127 SSRs identified in silico.
Method / resource The clove genome SSR overall frequency is 514.25 loci/Mb.
Observation Mononucleotide repeats were the most abundant repeat type in the clove genome (50.30%).
Observation Dinucleotide repeats were the most abundant repeat type in the clove genome (40.68%).
Observation The clove SSRs show a strong A/T-rich bias.
Observation The clove SSRs show a notable scarcity of CG/GC motifs.
Observation SSRs are uniformly distributed at the whole-chromosome level in clove.
Observation SSR density shows significant intra-chromosomal heterogeneity with hotspots and coldspots in clove.
Observation The clove genome has 11,107 hypervariable Class I SSR loci.
Method / resource 28,147 high-quality primer pairs targeting Class I and II SSRs were developed for clove.
Method / resource Validated clove SSR primer pairs showed 96.4% amplification success on 19 germplasm accessions.
Method / resource Validated clove SSR primer pairs showed 90.6% polymorphism on 19 germplasm accessions.
Observation The validated clove markers show moderate genetic diversity (mean He 0.22).
Observation The validated clove markers show a PIC of 0.18, indicating moderate genetic diversity.
Observation Phylogenetic analysis resolved the 19 clove accessions into distinct clades.
Observation One clove accession (Acc. 9833) with red-coloured flower buds is highly divergent.
Observation The validated clove markers were useful for hybrid authentication.
Observation Co-dominant Mendelian segregation was confirmed in a clove F2 population.
Observation A publicly accessible, searchable Clove SSR Marker Database was built.