Arctotheca calendula 175 claims

Claim · model-written summary

EPSPS transcript sequencing revealed two partial sequences of 1001 bp and 998 bp in Arctotheca calendula.

Taxon

Arctotheca calendulaArctotheca calendulat0

Gene

EPSPSt0

Outcome

partial sequences of the EPSPS transcriptse1

Value

1001 bp and 998 bpe1

Verbatim

t0

“EPSPS target site mechanisms confer glyphosate resistance in Arctotheca calendula”

e1

“Target‐site EPSPS gene sequencing revealed two partial sequences of the EPSPS transcripts (1001 bp and 998 bp)”

Claim · model-written summary

A target‐site mutation resulting in a Pro‐106‐Ser substitution was identified in the resistant Arctotheca calendula plants.

Taxon

Arctotheca calendulaArctotheca calendulat0

Gene

EPSPSt0

Outcome

target‐site mutatione2

Qualifier

Pro‐106‐Ser substitutione2

Verbatim

t0

“EPSPS target site mechanisms confer glyphosate resistance in Arctotheca calendula”

e2

“A known resistance‐endowing target‐site mutation in the 1551‐bp transcript was identified in the resistant plants, resulting in the Pro‐106‐Ser substitution”

Claim · model-written summary

The subpopulation from the mutant plants showed higher resistance to glyphosate than the susceptible population.

Taxon

Arctotheca calendulaArctotheca calendulat0

Treatment

glyphosatee3

Outcome

resistance to glyphosatee3

Direction

higherinferrede3

Value

>10‐folde3

Comparator

susceptible populatione3

Verbatim

t0

“EPSPS target site mechanisms confer glyphosate resistance in Arctotheca calendula”

e3

“The subpopulation derived from these mutant plants exhibited >10‐fold resistance to glyphosate compared to the susceptible population”

Claim · model-written summary

The EPSPS gene was constitutively expressed at a higher level in the resistant than in the susceptible populations.

Taxon

Arctotheca calendulaArctotheca calendulat0

Gene

EPSPSe4

Outcome

constitutively expressede4

Direction

highere4

Value

4.3‐folde4

Comparator

susceptible populationse4

Verbatim

t0

“EPSPS target site mechanisms confer glyphosate resistance in Arctotheca calendula”

e4

“the EPSPS gene (1551 bp) was constitutively expressed at a higher level (4.3‐fold) in the resistant than in the susceptible populations”

Claim · model-written summary

Foliar uptake of 14C-glyphosate was similar between resistant and susceptible capeweed.

Taxon

Arctotheca calendulaArctotheca calendulat0

Treatment

glyphosatee5

Outcome

foliar uptakee5

Direction

similare5

Comparator

susceptible populatione5

Verbatim

t0

“EPSPS target site mechanisms confer glyphosate resistance in Arctotheca calendula”

e5

“14 C‐glyphosate foliar uptake was similar with no visual difference in 14 C‐glyphosate translocation from leaves to other parts of a plant, between the resistant and susceptible population”

Claim · model-written summary

Translocation of 14C-glyphosate from leaves to other parts of the plant showed no visual difference between resistant and susceptible capeweed.

Taxon

Arctotheca calendulaArctotheca calendulat0

Treatment

glyphosatee5

Outcome

translocation from leaves to other parts of a plante5

Direction

no visual differencee5

Comparator

susceptible populatione5

Verbatim

t0

“EPSPS target site mechanisms confer glyphosate resistance in Arctotheca calendula”

e5

“14 C‐glyphosate foliar uptake was similar with no visual difference in 14 C‐glyphosate translocation from leaves to other parts of a plant, between the resistant and susceptible population”

Claim · model-written summary

Two partial EPSPS transcripts of 1001 bp and 998 bp were found, and a 1551 bp sequence was cloned from resistant plants.

Taxon

Arctotheca calendulaArctotheca calendulat0

Gene

EPSPSe0

Outcome

two partial sequences of the EPSPS transcriptse0

Value

1001 bp and 998 bpe0

Verbatim

t0

“Arctotheca calendula”

e0

“Target-site EPSPS gene sequencing revealed two partial sequences of the EPSPS transcripts (1001 bp and 998 bp), and the full-length sequence (1551 bp) containing the 1001-bp transcript was cloned as it was found in the resistant plants”

Claim · model-written summary

The resistant plants carried a target site mutation causing Pro-106-Ser substitution.

Taxon

Arctotheca calendulaArctotheca calendulat0

Gene

Pro-106-Sere0

Outcome

target-site mutatione0

Not reportedDirection

Verbatim

t0

“Arctotheca calendula”

e0

“A known resistance-endowing target-site mutation in the 1551-bp transcript was identified in the resistant plants, resulting in the Pro-106-Ser substitution”

Claim · model-written summary

Mutant-derived plants had more than tenfold glyphosate resistance versus the susceptible population.

Taxon

Arctotheca calendulaArctotheca calendulat0

Material

mutant plantse0

Outcome

resistance to glyphosatee0

Direction

>10-folde0

Value

>10-folde0

Comparator

susceptible populatione0

Verbatim

t0

“Arctotheca calendula”

e0

“The subpopulation derived from these mutant plants exhibited >10-fold resistance to glyphosate compared to the susceptible population”

Claim · model-written summary

EPSPS expression was 4.3-fold higher in resistant than susceptible populations.

Taxon

Arctotheca calendulaArctotheca calendulat0

Material

resistante0

Gene

EPSPSe0

Outcome

constitutively expressede0

Direction

highere0

Value

4.3-folde0

Comparator

susceptible populationse0

Verbatim

t0

“Arctotheca calendula”

e0

“the EPSPS gene (1551 bp) was constitutively expressed at a higher level (4.3-fold) in the resistant than in the susceptible populations”