Abstract
Phosphoenolpyruvate Carboxykinase, encoded by the pepck gene, plays an important role in gluconeogenesis. It also seems to be important in metabolism of nitrogenous compounds in developing seeds of legumes, including amides and ureides which are then transformed into amino acids, necessary for the synthesis of storage proteins. In this research, pepck gene expression in mRNA level, in different genotypes of chickpea (Cicer arietinum L.), was determined. Two low protein genotypes (MCC291 and MCC373) and two high protein genotypes (MCC458 and MCC053) out of 20 chickpea genotypes were selected. Total RNA were extracted through different stages of seed development, and the expression of the pepck gene was estimated by semi-quantitative RT-PCR. The results of the RT-PCR showed that two isoforms of this gene are expressed in high protein genotypes, whereas in the low protein genotypes, the expression of these isoforms was not obvious. Also this method showed a differential expression of pepck gene in different stages of flowering and seed development. pepck gene is expressed in higher levels during the sheet formation and developing seeds compared to the flowering and seed formation stages. Probably, the differential expression of pepck gene is related to its possible role in metabolism of seed components, particularly in determination of the protein content of chickpea seeds.
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Claims 4
Claim · model-written summary
Two pepck isoforms were expressed in high protein chickpea genotypes, but were not obvious in low protein genotypes.
- Taxon
- chickpea
Cicer arietinumt0 - Material
- high protein genotypese0
- Gene
- pepckt0
- Outcome
- two isoformse0
- Comparator
- low protein genotypese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two isoforms of this gene are expressed in high protein genotypes, whereas in the low protein genotypes, the expression of these isoforms was not obvious.”
t0“pepck gene expression in developing seeds and leaves of chickpea”
Claim · model-written summary
pepck expression differed across flowering and seed developmental stages.
- Taxon
- chickpeaCicer arietinumt0
- Organ / stage
- flowering and seed developmente0
- Gene
- pepcke0
- Outcome
- differential expression of pepck genee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a differential expression of pepck gene in different stages of flowering and seed development.”
t0“chickpea”
Claim · model-written summary
pepck expression was higher during sheet formation and developing seeds than flowering and seed formation.
- Taxon
- chickpeaCicer arietinumt0
- Organ / stage
- sheet formation and developing seedse0
- Gene
- pepcke0
- Outcome
- pepck genee0
- Direction
- higher levelse0
- Comparator
- flowering and seed formation stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pepck gene is expressed in higher levels during the sheet formation and developing seeds compared to the flowering and seed formation stages.”
t0“chickpea”
Claim · model-written summary
The authors suggest pepck expression may be related to chickpea seed protein content.
- Taxon
- chickpeaCicer arietinume0
- Gene
- pepcke0
- Outcome
- protein content of chickpea seedse0
- Qualifier
- Probablye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Probably, the differential expression of pepck gene is related to its possible role in metabolism of seed components, particularly in determination of the protein content of chickpea seeds.”