Abstract
The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination. Dry, high-vigor axes contained 1.2 [plus or minus] 0.1, nmol/axis and after an increase during the initial 6 h of imbibition, there was a decline to 1.0 [plus or minus] 0.1 nmol/axis at 24 h of incubation. Incubation in the presence of the redox-cycling agent paraquat (4 mM) for 24 h increased the [alpha]T content to 1.9 [plus or minus] 0.2 nmol/axis. When the incubation medium was supplemented with 500 [mu]M Fe-EDTA over 24 h, the content of [alpha]T increased to 1.8 [plus or minus] 0.1 nmol/axis. Isolated axes from soybean seeds stored for 56 months contained 6.5 [plus or minus] 0.3 nmol of [alpha]T/axis after 24 h of imbibition as compared to 1.0 [plus or minus] 0.1 nmol of [alpha]T/axis in axes from soybean seeds stored for 8 months. In all of these experimental situations, oxidant production as assessed in vivo by a fluorometric assay was increased by 4 mM paraquat (8-fold), 500 [mu]M iron (2-fold), and 56 months of storage (4-fold) after 24 h of imbibition. The data presented here suggest that the cellular content of [alpha]T is physiologically adjusted as a response to conditions of oxidative stress.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Dry, high-vigor soybean embryonic axes contained 1.2 +/- 0.1 nmol alpha-tocopherol per axis.
- Taxon
- soybean
Glycine maxe1 - Material
- var Hoode1
- Organ / stage
- embryonic axese1
- Outcome
- content of [alpha]-tocopherole1
- Value
- 1.2 [plus or minus] 0.1, nmol/axise2
- Qualifier
- Dry, high-vigore2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e2“Dry, high-vigor axes contained 1.2 [plus or minus] 0.1, nmol/axis”
Claim · model-written summary
Alpha-tocopherol content of soybean embryonic axes increased during the initial 6 h of imbibition.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- embryonic axese1
- Outcome
- content of [alpha]-tocopherole1
- Direction
- increasee2
- Qualifier
- during the initial 6 h of imbibitione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e2“after an increase during the initial 6 h of imbibition”
Claim · model-written summary
Alpha-tocopherol content of soybean embryonic axes declined to 1.0 +/- 0.1 nmol per axis at 24 h of incubation.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- embryonic axese1
- Outcome
- content of [alpha]-tocopherole1
- Direction
- declinee3
- Value
- 1.0 [plus or minus] 0.1 nmol/axise3
- Qualifier
- at 24 h of incubatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e3“there was a decline to 1.0 [plus or minus] 0.1 nmol/axis at 24 h of incubation”
Claim · model-written summary
Paraquat (4 mM) for 24 h increased alpha-tocopherol content of soybean embryonic axes to 1.9 +/- 0.2 nmol per axis.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- embryonic axese1
- Treatment
- redox-cycling agent paraquat (4 mM) for 24 he4
- Outcome
- [alpha]T contente4
- Direction
- increasede4
- Value
- 1.9 [plus or minus] 0.2 nmol/axise4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e4“Incubation in the presence of the redox-cycling agent paraquat (4 mM) for 24 h increased the [alpha]T content to 1.9 [plus or minus] 0.2 nmol/axis”
Claim · model-written summary
Supplementing the incubation medium with 500 uM Fe-EDTA over 24 h increased alpha-tocopherol content of soybean embryonic axes to 1.8 +/- 0.1 nmol per axis.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- embryonic axese1
- Treatment
- 500 [mu]M Fe-EDTA over 24 he5
- Outcome
- content of [alpha]Te5
- Direction
- increasede5
- Value
- 1.8 [plus or minus] 0.1 nmol/axise5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e5“When the incubation medium was supplemented with 500 [mu]M Fe-EDTA over 24 h, the content of [alpha]T increased to 1.8 [plus or minus] 0.1 nmol/axis”
Claim · model-written summary
After 24 h of imbibition, soybean axes from seeds stored for 56 months contained 6.5 +/- 0.3 nmol alpha-tocopherol per axis, higher than 1.0 +/- 0.1 nmol in axes from seeds stored for 8 months.
- Taxon
- soybeanGlycine maxe6
- Organ / stage
- Isolated axese6
- Treatment
- seeds stored for 56 monthse6
- Outcome
- [alpha]T/axise6
- Direction
- higherinferrede6
- Value
- 6.5 [plus or minus] 0.3 nmol of [alpha]T/axise6
- Qualifier
- after 24 h of imbibitione6
- Comparator
- axes from soybean seeds stored for 8 monthse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e6“Isolated axes from soybean seeds stored for 56 months contained 6.5 [plus or minus] 0.3 nmol of [alpha]T/axis after 24 h of imbibition as compared to 1.0 [plus or minus] 0.1 nmol of [alpha]T/axis in axes from soybean seeds stored for 8 months”
Claim · model-written summary
Paraquat (4 mM) increased in vivo oxidant production in soybean embryonic axes 8-fold after 24 h of imbibition.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- embryonic axese1
- Treatment
- 4 mM paraquate7
- Outcome
- oxidant productione7
- Direction
- increasede7
- Value
- 8-folde7
- Qualifier
- as assessed in vivo by a fluorometric assaye7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e7“oxidant production as assessed in vivo by a fluorometric assay was increased by 4 mM paraquat (8-fold)”
Claim · model-written summary
500 uM iron increased in vivo oxidant production in soybean embryonic axes 2-fold after 24 h of imbibition.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- embryonic axese1
- Treatment
- 500 [mu]M irone7
- Outcome
- oxidant productione7
- Direction
- increasede7
- Value
- 2-folde7
- Qualifier
- as assessed in vivo by a fluorometric assaye7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e7“oxidant production as assessed in vivo by a fluorometric assay was increased by 4 mM paraquat (8-fold), 500 [mu]M iron (2-fold)”
Claim · model-written summary
56 months of seed storage increased in vivo oxidant production in soybean embryonic axes 4-fold after 24 h of imbibition.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- embryonic axese1
- Treatment
- 56 months of storagee7
- Outcome
- oxidant productione7
- Direction
- increasede7
- Value
- 4-folde7
- Qualifier
- after 24 h of imbibitione7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e7“oxidant production as assessed in vivo by a fluorometric assay was increased by 4 mM paraquat (8-fold), 500 [mu]M iron (2-fold), and 56 months of storage (4-fold) after 24 h of imbibition”
Claim · model-written summary
The data suggest that cellular alpha-tocopherol content in soybean is physiologically adjusted in response to oxidative stress conditions.
- Taxon
- soybeanGlycine maxe1
- Treatment
- conditions of oxidative stresse7
- Outcome
- cellular content of [alpha]Te7
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination”
e7“The data presented here suggest that the cellular content of [alpha]T is physiologically adjusted as a response to conditions of oxidative stress”