Abstract
Different crossing techniques,emasculation and pollination procedures for obtaining young embryos from hybrid of Oryza minuta and Oryza sativa have been evaluated,and the effects of different sterilization methods(20% NaClO or 0.1% HgCl2),inoculation materials,culture media and the age of embryos on the germination rate of young embryo has been investigated by using four cultivated rice(Oryza sativa,2n=24,AA) and two wild specie(Oryza minuta,2n=48,BBCC).The results showed that the production of young embryo from hybrids was higher when cultivated rice was used as female and wild species as male parent.Emasculation with artificial spikelet-cut and continuous pollination for 3 days resulted in to higher rates of embryo development.During the process of embryo rescue,applying young embryo after pollinating of 10-12 days and sterilizing with 0.1% HgCl2 for 10 minutes followed by culture inoculation with ovary-slice to 1 / 4 MS medium resulted in the higher germination rate of young embryo,and the hybrid plant were obtained.These methods are simple,convenient and economically feasible,therefore,may be utilized in crossing of cultivated rice and wild species without AA genome.
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Claims 6
Claim · model-written summary
Using cultivated rice as the female and wild rice as the male increased hybrid embryo production.
- Taxon
- Oryza sativa
Oryza sativat0
- Material
- Oryza minutat0
- Treatment
- cultivated rice was used as female and wild species as male parente0
- Outcome
- production of young embryo from hybridse0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“production of young embryo from hybrids was higher when cultivated rice was used as female and wild species as male parent”
t0“Oryza minuta and Oryza sativa”
Claim · model-written summary
Artificial spikelet-cut emasculation and continuous pollination for three days raised embryo development.
- Taxon
- Oryza sativa
Oryza sativat0
- Treatment
- Emasculation with artificial spikelet-cut and continuous pollination for 3 dayse0
- Outcome
- rates of embryo developmente0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Emasculation with artificial spikelet-cut and continuous pollination for 3 days resulted in to higher rates of embryo development”
t0“Oryza minuta and Oryza sativa”
Claim · model-written summary
Embryos at 10–12 days, sterilized with HgCl2 and cultured with ovary-slice on quarter-strength MS medium, had higher germination.
- Taxon
- Oryza sativa
Oryza sativat0
- Treatment
- applying young embryo after pollinating of 10-12 days and sterilizing with 0.1% HgCl2 for 10 minutes followed by culture inoculation with ovary-slice to 1 / 4 MS mediume0
- Outcome
- germination rate of young embryoe0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“applying young embryo after pollinating of 10-12 days and sterilizing with 0.1% HgCl2 for 10 minutes followed by culture inoculation with ovary-slice to 1 / 4 MS medium resulted in the higher germination rate of young embryo”
t0“Oryza minuta and Oryza sativa”
Claim · model-written summary
The embryo rescue procedure produced hybrid plants.
- Taxon
- Oryza sativa
Oryza sativat0
- Material
- Oryza minutat0
- Outcome
- hybrid plant were obtainede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the hybrid plant were obtained”
t0“Oryza minuta and Oryza sativa”
Claim · model-written summary
The authors consider the crossing methods simple, convenient and economically feasible.
- Taxon
- Oryza sativa
Oryza sativat0
- Outcome
- simple,convenient and economically feasiblee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These methods are simple,convenient and economically feasible”
t0“Oryza minuta and Oryza sativa”
Claim · model-written summary
The authors recommend the methods for crossing cultivated rice with wild species lacking an AA genome.
- Taxon
- Oryza sativa
Oryza sativat0
- Outcome
- crossing of cultivated rice and wild species without AA genomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“may be utilized in crossing of cultivated rice and wild species without AA genome”
t0“Oryza minuta and Oryza sativa”
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