Paper · 2010 · Guangxi nongye kexue

Discussions on wide-crossing between Oryza minuta and Oryza sativa.

Sibin Guo; Xinghua Lin

6
claims
12
verbatim quotes
1
citations
3
research areas
—
Not open access

Abstract

Different crossing techniques,emasculation and pollination procedures for obtaining young embryos from hybrid of Oryza minuta and Oryza sativa have been evaluated,and the effects of different sterilization methods(20% NaClO or 0.1% HgCl2),inoculation materials,culture media and the age of embryos on the germination rate of young embryo has been investigated by using four cultivated rice(Oryza sativa,2n=24,AA) and two wild specie(Oryza minuta,2n=48,BBCC).The results showed that the production of young embryo from hybrids was higher when cultivated rice was used as female and wild species as male parent.Emasculation with artificial spikelet-cut and continuous pollination for 3 days resulted in to higher rates of embryo development.During the process of embryo rescue,applying young embryo after pollinating of 10-12 days and sterilizing with 0.1% HgCl2 for 10 minutes followed by culture inoculation with ovary-slice to 1 / 4 MS medium resulted in the higher germination rate of young embryo,and the hybrid plant were obtained.These methods are simple,convenient and economically feasible,therefore,may be utilized in crossing of cultivated rice and wild species without AA genome.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Using cultivated rice as the female and wild rice as the male increased hybrid embryo production.

Taxon
Oryza sativaOryza sativat0
Material
Oryza minutat0
Treatment
cultivated rice was used as female and wild species as male parente0
Outcome
production of young embryo from hybridse0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“production of young embryo from hybrids was higher when cultivated rice was used as female and wild species as male parent”

✓ located verbatim in the source · abstract, characters 486–608 · show in text ↑
t0

“Oryza minuta and Oryza sativa”

✓ located verbatim in the source · title, characters 37–66 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Artificial spikelet-cut emasculation and continuous pollination for three days raised embryo development.

Taxon
Oryza sativaOryza sativat0
Treatment
Emasculation with artificial spikelet-cut and continuous pollination for 3 dayse0
Outcome
rates of embryo developmente0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Emasculation with artificial spikelet-cut and continuous pollination for 3 days resulted in to higher rates of embryo development”

✓ located verbatim in the source · abstract, characters 609–738 · show in text ↑
t0

“Oryza minuta and Oryza sativa”

✓ located verbatim in the source · title, characters 37–66 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Embryos at 10–12 days, sterilized with HgCl2 and cultured with ovary-slice on quarter-strength MS medium, had higher germination.

Taxon
Oryza sativaOryza sativat0
Treatment
applying young embryo after pollinating of 10-12 days and sterilizing with 0.1% HgCl2 for 10 minutes followed by culture inoculation with ovary-slice to 1 / 4 MS mediume0
Outcome
germination rate of young embryoe0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“applying young embryo after pollinating of 10-12 days and sterilizing with 0.1% HgCl2 for 10 minutes followed by culture inoculation with ovary-slice to 1 / 4 MS medium resulted in the higher germination rate of young embryo”

✓ located verbatim in the source · abstract, characters 775–999 · show in text ↑
t0

“Oryza minuta and Oryza sativa”

✓ located verbatim in the source · title, characters 37–66 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The embryo rescue procedure produced hybrid plants.

Taxon
Oryza sativaOryza sativat0
Material
Oryza minutat0
Outcome
hybrid plant were obtainede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the hybrid plant were obtained”

✓ located verbatim in the source · abstract, characters 1004–1034 · show in text ↑
t0

“Oryza minuta and Oryza sativa”

✓ located verbatim in the source · title, characters 37–66 · show in text ↑
Observationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors consider the crossing methods simple, convenient and economically feasible.

Taxon
Oryza sativaOryza sativat0
Outcome
simple,convenient and economically feasiblee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“These methods are simple,convenient and economically feasible”

✓ located verbatim in the source · abstract, characters 1035–1096 · show in text ↑
t0

“Oryza minuta and Oryza sativa”

✓ located verbatim in the source · title, characters 37–66 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommend the methods for crossing cultivated rice with wild species lacking an AA genome.

Taxon
Oryza sativaOryza sativat0
Outcome
crossing of cultivated rice and wild species without AA genomee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“may be utilized in crossing of cultivated rice and wild species without AA genome”

✓ located verbatim in the source · abstract, characters 1107–1188 · show in text ↑
t0

“Oryza minuta and Oryza sativa”

✓ located verbatim in the source · title, characters 37–66 · show in text ↑

mercury dichloride · growth · Oryza sativa (rice)Positive and negative results

6 papers: 2 positive, 4 negative or no effect

1 paper in the same direction as this paper · 4 in the opposite direction

  1. no effect
  2. negative
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maleate(2-) · yield · Oryza sativa (rice)Positive and negative results

9 papers: 6 positive, 3 negative or no effect

5 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. negative
  3. negative

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in vitro · growth · Oryza sativa (rice)Positive and negative results

165 papers: 140 positive, 25 negative or no effect

139 papers in the same direction as this paper · 24 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

161 papers more in this group

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inoculation · growth · Oryza sativa (rice)Positive and negative results

399 papers: 362 positive, 37 negative or no effect

361 papers in the same direction as this paper · 34 in the opposite direction · 3 reporting no difference from an alternative

  1. no effect
  2. negative
  3. no effect

395 papers more in this group

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pollination · growth · Oryza sativa (rice)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.